Figure 6. Short-term metabolic changes in bP23H retinas. A–I, Visualization of metabolic content and distribution by quantitative metabolite mapping on 90 nm sections probed with Igs specific to each metabolite and visualized with silver intensification. J–L, Red–green–blue channels represent taurine–glutathione–glutamate mapping. The pink and red compartments in the photoreceptor outer segments (OS) and inner segments (IS) contain distinct taurine–glutamate–glutathione mixtures, whereas various blue-to-azure cells in the interneuron layer (INL) and ganglion cell layer (GCL) are neurons with distinctive glutamate content. The green–yellow background of RPE reflect high levels of glutathione. M–O, Theme maps display results of multispectral analyses and clustering analyses to extract all distinct molecular phenotypes in the photoreceptor layer. Signals from glutamate, glutathione, taurine, aspartate, GABA, glycine, arginine, alanine, and CRALBP were used for multispectral analyses and clustering analyses. M, N, Theme maps show that the metabolic profiles of rods and cones in control and degenerating retina were similar; whereas in recovering retina, rods displayed variability in their metabolic profile because of variability in their taurine, glutamate, and glutathione content. Arrows and arrowheads indicate Müller glia and regenerating rods, respectively.