Article Information
- Received May 14, 2012
- Revision received January 22, 2013
- Accepted January 25, 2013
- First published March 27, 2013.
- Version of record published March 27, 2013.
Author Information
Author contributions
Author contributions: V.D., I.M.H., and R.S.Z. designed research, V.D. and I.M.H. performed research, V.D., I.M.H., and R.S.Z. analyzed data, and R.S.Z. wrote the paper.
Disclosures
- Received May 14, 2012.
- Revision received January 22, 2013.
- Accepted January 25, 2013.
This research was supported by the National Institutes of Health (Grants R21 NS048016 and R01 NS054760) and the National Science Foundation (Grant SGER 0332968). We thank Ed Levitan, Ed Chapman, Richard Kramer, Ehud Isacoff, Manfred Lindau, and Doris Fortin for discussion, Russell English for technical assistance, Christophe Hansen-Estruch for assistance with data analysis, Arunesh Saras and Sarit Ahituv for assistance with molecular biology and for advice, Ed Chapman for providing EYFP-SNAP-25B and ECFP-VAMP-2, Roger Tsien for the gift of citrine, David Piston for the gift of monomeric cerulean, and Mark Rizzo for the gifts of cerulean-3-VAMP-2 and agarose beads coated with cerulean-venus or cerulean-3-venus dimers, and Kevin Staras for help in developing the initial protocols and performing the preliminary experiments.
The authors declare no competing financial interests.
- Correspondence should be addressed to Robert S. Zucker, Department of Molecular and Cell Biology, 111 Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720. zucker{at}berkeley.edu
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