Figure 3. Hypomyelination was observed in the brains of juvenile mice exposed to E. coli as neonates. Neonatal mice (P3) were injected subcutaneously with either saline (CON) or E. coli (ELI), and brain tissue from male and female mice was collected and stained for myelin 1 month later (P35). Representative images and quantification of myelin staining are shown in: A, coronal section and B, quantification of total white and gray matter areas examined; C, D, the mid-section of the corpus callosum; E, F, the cingulum bundle of the corpus callosum; G, H, the motor cortex; and I, J, cerebellum. Bars represent the mean ± SEM (n = 6–9). Means with asterisk are significantly different from controls (p < 0.05), and means with plus sign tended to be different from controls (p ≤ 0.1). Rel, Relative; CC, Corpus callosum; Cing, cingulum bundle; MCX, motor cortex; CBM, cerebellum. Scale bars: A, C, 500 μm; E, G, I, 250 μm.