Figure 8. Neocortical expression of RZRβ, Cad8, and Id2. Coronal sections (100 μm) of P0 control and PrEE brain hemispheres following free-floating nonradioactive in situ hybridization with a probe against RZRβ (A1, A2, B1, B2), Cad8 (C1, C2) or Id2 (D1, D2). A1, Section through a caudal region of frontal cortex where left arrow denotes low medial cortical expression of RZRβ and a right arrow denotes moderate lateral expression in a control case. A2, Section through a caudal region of frontal cortex where left arrow denotes moderate medial cortical expression of RZRβ and a right arrow denotes high lateral expression in a PrEE case. B1, Section through a rostral region of parietal cortex where left black arrow denotes low medial cortical expression of RZRβ, a white middle arrow denotes the medial boundary of RZRβ expression, and a right arrow denotes moderate lateral expression of RZRβ in a control case. B2, Section through a rostral region of parietal cortex where left black arrow denotes moderate medial cortical expression of RZRβ, a white middle arrow denotes the shifted medial boundary of RZRβ expression and a right arrow denotes high lateral expression of RZRβ in a PrEE case. C1, Section through a rostral region of parietal cortex where black arrow denotes lateral boundary of Cad8 expression in a control case. C2, Section through a rostral region of parietal cortex where black arrow denotes a shifted lateral boundary of Cad8 expression in a PrEE case. D1, Section through a rostral region of parietal cortex where black arrow denotes an absence of Id2 expression within cortical layers 3 and 4 in a control case. D2, Section through a rostral region of parietal cortex where black arrow denotes an extension of the lateral Id2 expression within layers 3/4. E1, E2, Patterns of gene expression compressed onto a coronal reconstruction of control (E1) or PrEE (E2) brains. Patterned areas represent gene expression as follows: RZRβ, diagonal line area; Cad8, crossed line area; Id2, dotted area. Dark line, cortical outline. Sections oriented dorsal (D) up and lateral (L) right.