Figure 8. Simultaneous inhibition of calmodulin and MLCK does not induce additive inhibition of endocytosis. A, Sampled Cm changes induced by depol20ms from calyces dialyzed with the pipette solution containing CBD (500 μm) along with or without MLCKip (2 μm). B, Averaged Cm responses induced by depol20ms in control (n = 10), CBD (n = 8), MLCKip (n = 10), and CBD with MLCKip (n = 8), with the error bars indicating SEM. Individual Cm traces were normalized by ΔCm before averaging. Data of control and MLCKip are from the same tests as shown in Figure 1. C, Comparison of ΔCm, Rate_endo, and ΔCm25s induced by depol20ms. D, Sampled Cm changes induced by depol20ms×10 from calyces dialyzed with CBD and/or MLCKip. E, Averaged Cm responses induced by depol20ms×10 in control (n = 10), CBD (n = 7), MLCKip (n = 10), and CBD with MLCKip (n = 6), which are indicated by the same colors as in B. Data of control and MLCKip are from the same tests as shown in Figure 4. Individual Cm traces were normalized by ΔCm before averaging. F, Comparison of ΔCm, Rate_endo and ΔCm25s induced by depol20ms×10.