Figure 2. GD65 restrains synaptic growth at the larval NMJ. A, Representative confocal images of muscle 4 NMJ synapses demonstrating a large increase in synaptic branches and synaptic boutons in GD65 mutants (axons in red, α-HRP; boutons green, α-DVGLUT). The graphs show quantification of bouton number (22 vs 46, *p < 0.05), bouton size (9.68 vs 3.82 μm2), ***p < 0.001), and branch number (2.6 vs 6.4, **p < 0.01) for WT and the GD65 mutants (n = 12). B, GD65 mutants exhibit a strong maternal effect on synaptic growth. Maternal and zygotic genotypes are listed for each genotype tested. Quantification of bouton number from motoneuron MN4b on muscle 4 is shown. Bouton number = 20.3, 50.4, 25.8, 32.4, 22.1, 20.5, and N = 16, 15, 15, 13, 16, 14 respectively for each genotype. Error bars indicate ±SEM; **p < 0.01.