Article Information
- Received January 15, 2014
- Revision received June 18, 2014
- Accepted July 9, 2014
- First published August 13, 2014.
- Version of record published August 13, 2014.
Author Information
- Frederik Baumkötter1,
- Nadine Schmidt1,
- Carolyn Vargas2,
- Sandra Schilling1,
- Rebecca Weber1,
- Katja Wagner1,
- Sebastian Fiedler2,
- Wilfried Klug4,
- Jens Radzimanowski5,
- Sebastian Nickolaus3,
- Sandro Keller2,
- Simone Eggert1,
- Klemens Wild4, and
- Stefan Kins1
- 1Division of Human Biology and Human Genetics,
- 2Molecular Biophysics and
- 3Division of Plant Physiology, University of Kaiserslautern, D-67663 Kaiserslautern, Germany,
- 4Heidelberg University Biochemistry Center, University of Heidelberg, D-69120 Heidelberg, Germany, and
- 5Unit of Virus Host Cell Interactions, International Coeducational Unit 3265, Joseph Fourier University–European Molecular Biology Laboratory–National Center of Scientific Research, F-38042 Grenoble, France
Author contributions
Author contributions: F.B., S.Ke., S.E., K.Wi., and S.Ki. designed research; F.B., N.S., C.V., S.S., R.W., K.Wa., S.F., W.K., J.R., and S.N. performed research; F.B., N.S., C.V., S.S., R.W., K.Wa., S.F., J.R., S.Ke., S.E., K.Wi., and S.Ki. analyzed data; F.B., S.Ke., K.Wi., and S.Ki. wrote the paper.
Disclosures
- Received January 15, 2014.
- Revision received June 18, 2014.
- Accepted July 9, 2014.
This work was supported by the Fritz Thyssen Foundation (S.Ki.) and the German Research Foundation, including the Research Group FOR1332 (S.Ki. and K.Wi.) and the Rheinland-Pfalz Foundation for Innovation (S.Ki. and S.Ke.). We thank L. Hanke for administrative support, D. Gross for excellent technical assistance, and J. Klement for help in purifying APP fusion proteins. We thank C. Tischer (European Molecular Biology Laboratory, Heidelberg, Germany) for help with cell profiler and immunocytochemical quantification analysis, J. Kopp and C. Siegmann from the crystallization platform of the Heidelberg University Biochemistry Center/Cluster of Excellence: CellNetworks for help in protein crystallization, and I. Sinning (Heidelberg University Biochemistry Center, Heidelberg, Germany) for generous support. We acknowledge the European Synchrotron Radiation Facility for providing synchrotron radiation and for assistance in using beam-lines ID23-2 and BM29. We thank A. Vest and P. Scheiffele for help with the hemisynapse assay and P. Scheiffele for providing the Neuroligin-1 cDNA.
The authors declare no competing financial interests.
- Correspondence should be addressed to either of the following: Stefan Kins, Division of Human Biology and Human Genetics, University of Kaiserslautern, Erwin-Schrödinger-Straße 13, D-67663 Kaiserslautern, Germany, s.kins{at}biologie.uni-kl.de; or Klemens Wild, Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, University of Heidelberg, D-69120 Heidelberg, Germany. klemens.wild{at}bzh.uni-heidelberg.de
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