Figure 3. Dα7 and Dα6 levels decreases, but DLG level increases in the synaptic regions of adult higdd37 brain. A, Reduction of Dα7 localization at synapses of the optic lobe (top) and calyx microglomeruli (bottom) in higdd37. Hig was well colocalized with Dα7. B, Decrease of Dα6 localization at subsets of synapses in the optic lobe of higdd37. Magnified views of dotted areas of the medulla are shown in the middle panels for WT, and in the bottom center panel for higdd37. Strong Hig staining is found in specific layers of synapses that also contain Dα6 signals (arrowheads). Dα6 in these synapses (bracket) is significantly decreased in higdd37. Dα6 signals disappeared in Dα6DAS1 mutant brains, confirming the specificity of the anti-Dα6 antibody. C, Reduction in Dα6 signals at the synapses of the calyx microglomeruli in higdd37. D–F, Abnormal accumulation of DLG in higdd37. D, Frontal optical sections of WT and higdd37 brains stained for DLG, which labels synapses. The dotted square in the synaptic region is enlarged in E. E, Abnormal accumulation of DLG at cholinergic synapses in higdd37. Strong DLG signals (arrowheads) are adjacent to the presynaptic marker Syt-GFP, expressed under the control of cha-Gal4 (dotted squares, and insets for magnified views). Scale bar: E, inset, 1 μm. F, Abnormal accumulation of DLG in the calyx of higdd37. The intensity of DN-cadherin in higdd37 is comparable with that in the WT. G, Quantitative analyses of synaptic proteins Dα7, Dα6, DLG, and DN-cadherin in the calyx of WT, higdd37, and rescued flies (elav-Gal4/Y; higdd37; UAS-hig-GFP/+). Intensity of fluorescence in higdd37 is normalized to the WT values. Error bars indicate ± SEM. **p < 0.01, compared with WT (ANOVA followed by Bonferroni's post hoc test). NS, Not significantly different.