Figure 1. Viral expression of DmOctβR1 is restricted to excitatory neurons of the hippocampus. A, Mice were injected with pAAV9-CaMKIIα0.4-EGFP or pAAV9-CaMKIIα0.4-DmOctβ1R-HA to drive expression of EGFP or the Gαs-coupled DmOctβ1R in hippocampal excitatory neurons. B, Activation of the DmOctβ1R by its ligand octopamine (OCT) stimulates adenylyl cyclase activity, which synthesizes cAMP. C, D, Representative low-magnification images of EGFP and DmOctβ1R expression in coronal brain sections. Scale bar, 570 μm. E, F, Representative images EGFP and DmOctβ1R expression in all three major hippocampal subregions. Scale bar, 200 μm. G–L, Higher-magnification images of area CA1 (E, H), CA3 (F, I), and dentate gyrus (G, J). Scale bar, 20 μm. AC, Adenyl cyclase; ITR, inverted terminal repeats.