Figure 10. Cell-autonomous effects of miR-155 on neural differentiation, survival, and migration molecules of NSCs in vitro. A, Representative images of NSCs transfected with mock transfection (lipofectamine only), scramble Cy3-labeled miR mimic, or pre-miR-155 mimic and cultured for 72 h in differentiation conditions. Green, DCX; red, GFAP; blue, DAPI. Original magnification = 20×. Scale bar, 50 μm. B, Percentage DCX+ (black bars) and GFAP+ (white bars) cells with whole nuclei (live cells at fixation time) out of total cells. C, Percentage of cells with fragmented nuclei (apoptotic cells) out of total cells. D, Cleaved caspase-3 fluorescence intensity in NSCs transfected with mock, control, or pre-miR-155 mimic (procedure defined units, p.d.u; B–D) at least 10 20× original magnification images counted/ measured per group. **p < 0.01 and *p < 0.05 as determined by 1-way ANOVA and Tukey post hoc. E, Transfection efficiency of Cy3-labeled control miR mimic. Green, Cy3; red, GFAP (astrocytes); blue, DAPI (nuclei). F, RT-PCR target gene expression analysis of NSCs transfected with control or pre-miR-155 mimic. Left, Primer set 1 (see Materials and Methods for sequences), Right, Primer set 2. **ΔΔCT p < 0.01 versus control, mimic-transfected NSCs; ###p < 0.001, ##p < 0.01, and #p < 0.05 versus mock-transfected NSCs as determined by 1-way ANOVA and Tukey post hoc. Data are shown as mean ± SEM.