Article Information
- Received March 20, 2015
- Revision received July 31, 2015
- Accepted August 10, 2015
- First published September 16, 2015.
- Version of record published September 16, 2015.
Author Information
- Goichi Miyoshi1,
- Allison Young1,
- Timothy Petros1,
- Theofanis Karayannis1,
- Melissa McKenzie Chang1,
- Alfonso Lavado2,
- Tomohiko Iwano3,
- Miho Nakajima4,
- Hiroki Taniguchi5,
- Z. Josh Huang5,
- Nathaniel Heintz4,
- Guillermo Oliver2,
- Fumio Matsuzaki3,
- Robert P. Machold1, and
- Gord Fishell1
- 1Department of Neuroscience and Physiology, NYU Neuroscience Institute, Smilow Research Center, New York University School of Medicine, New York, New York 10016,
- 2Department of Genetics & Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105,
- 3Laboratory for Cell Asymmetry, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan,
- 4Laboratory of Molecular Biology, Howard Hughes Medical Institute, GENSAT Project, The Rockefeller University, New York, New York 10065, and
- 5Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
Author contributions
Author contributions: G.M. designed research; G.M., A.Y., T.P., T.K., M.M.C., A.L., T.I., and G.F. performed research; G.M., M.N., H.T., Z.J.H., N.H., G.O., and F.M. contributed unpublished reagents/analytic tools; G.M., T.P., T.K., and M.M.C. analyzed data; G.M., R.P.M., and G.F. wrote the paper.
Disclosures
- Received March 20, 2015.
- Revision received July 31, 2015.
- Accepted August 10, 2015.
Research in the Fishell lab is supported by National Institutes of Health grants (PPG-NS309889, RO1NS039007, RO1NS081297, and RO1MH071679), the Simons Foundation, and New York State through the NYSTEM initiative. We thank Drs. Stewart Anderson and Sonia Garel for kindly sharing their protocols for embryonic brain slice electroporation and subsequent slice culture. We greatly appreciate the following for kindly sharing their reagents: Dr. Stewart Anderson (Nkx2-1BAC-Cre), Dr. Nicoletta Kessaris (Lhx6BAC-Cre), Dr. Ulrich Müeller (Wnt3a-Cre), Dr. Marc Ekker, and Dr. John Rubenstein (Dlx6a-Cre). Finally, we thank the past and present Fishell lab members for their support and constructive comments on this project and Lihong Yin for her technical help.
The authors declare no competing financial interests.
- Correspondence should be addressed to either Goichi Miyoshi or Gord Fishell, Department of Neuroscience and Physiology, NYU Neuroscience Institute, Smilow Research Center, New York University School of Medicine, 522 First Avenue, New York, NY 10016, Goichi.Miyoshi{at}gmail.com or fisheg01{at}nyumc.org
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