Abstract
The extracellular accumulation of amyloid β (Aβ) peptides is characteristic of Alzheimer's disease (AD). However, formation of diffusible, oligomeric forms of Aβ, both on and off pathways to amyloid fibrils, is thought to include neurotoxic species responsible for synaptic loss and neurodegeneration, rather than polymeric amyloid aggregates. The 8-hydroxyquinolines (8-HQ) clioquinol (CQ) and PBT2 were developed for their ability to inhibit metal-mediated generation of reactive oxygen species from Aβ:Cu complexes and have both undergone preclinical and Phase II clinical development for the treatment of AD. Their respective modes of action are not fully understood and may include both inhibition of Aβ fibrillar polymerization and direct depolymerization of existing Aβ fibrils. In the present study, we find that CQ and PBT2 can interact directly with Aβ and affect its propensity to aggregate. Using a combination of biophysical techniques, we demonstrate that, in the presence of these 8-HQs and in the absence of metal ions, Aβ associates with two 8-HQ molecules and forms a dimer. Furthermore, 8-HQ bind Aβ with an affinity of 1–10 μm and suppress the formation of large (>30 kDa) oligomers. The stabilized low molecular weight species are nontoxic. Treatment with 8-HQs also reduces the levels of in vivo soluble oligomers in a Caenorhabditis elegans model of Aβ toxicity. We propose that 8-HQs possess an additional mechanism of action that neutralizes neurotoxic Aβ oligomer formation through stabilization of small (dimeric) nontoxic Aβ conformers.
Introduction
Alzheimer's disease (AD) is characterized by the accumulation of the amyloid-β (Aβ) peptide within the extracellular space of the brain (Masters et al., 1985; Kang et al., 1987). Although these deposits of Aβ are the pathognomonic feature of AD, the degree of synaptic loss correlates more with the soluble/diffusible species of Aβ oligomers (Lambert et al., 1998), which in turn are in equilibrium with insoluble Aβ aggregates (Mawuenyega et al., 2010). This has led to the soluble oligomer theory of AD, where a specific small, diffusible Aβ oligomer causes synaptic damage beyond the brain's repair capacity. This theory has been supported by the finding of SDS-stable dimers in human brain tissue, which affect LTP (Shankar et al., 2008), and the observation that levels of a 56 kDa oligomer (labeled Aβ 56*) correlate with the severity of cognitive dysfunction (Lesné et al., 2006). There are many different methods for generating toxic oligomers in vitro (for review, see Benilova et al., 2012), which lead to a corresponding array of numerous oligomeric forms of Aβ, with little evidence of which in vitro configurations actually exist in vivo, or are specific to disease pathogenesis (for reviews, see Hayden and Teplow, 2013; Ryan et al., 2013b). Despite this uncertainty, assays of these oligomers and development of antibodies targeting the Aβ peptide have been a major theme in the recent efforts to develop a therapy for AD (Imbimbo et al., 2012; Tayeb et al., 2013).
Clioquinol (CQ; 5-chloro-7-iodo-quinolin-8-ol; Fig. 1A, inset), a first-generation metal-protein attenuating compound and 2-(dimethylamino)methyl-5,7-dichloro-8-hydroxyquinoline (PBT2; Figure 1A, inset) (Telpoukhovskaia et al., 2014), a second-generation metal-protein attenuating compound that crosses the blood–brain barrier (Padmanabhan et al., 1989; Adlard et al., 2008), are examples of alternative approaches to targeting Aβ in the brain. This approach uses small molecules directed toward inhibiting aberrant interactions of metals (e.g., Cu and Zn) and Aβ. PBT2 has successfully completed two Phase II clinical trials in AD, the first of which has shown PBT2 to have significant positive effects on cognition with concomitant reduction in CSF Aβ levels (Lannfelt et al., 2008; Faux et al., 2010). The results of the second Phase II trial are currently being evaluated.
The initial proposed mechanisms of action for PBT2 and CQ include a metal chaperone activity, inhibiting reactive oxygen species generated from Aβ:Cu complexes, inhibition of dityrosine cross-links, disaggregation of Aβ amyloid fibrils, and promoting uptake of metals by cells (Cherny et al., 2001; White et al., 2006; Adlard et al., 2008; Bush, 2008; Crouch et al., 2011). Two current studies have implicated the formation of ternary complexes between 8-hydroxyquinoline (8-HQ), Aβ, and a divalent metal cation in the proposed mechanism of action (Kenche et al., 2013; Matlack et al., 2014). It has also been observed that 8-HQs affect the aggregation of a range of proteins, including the Aβ peptide (LeVine et al., 2009; Cheng et al., 2012), Huntingtin polyglutamine, TDP-43, and α-synuclein (Tardiff et al., 2012) with no addition of exogenous metal ions. The direct, metal-independent interaction of 8-HQs with Aβ may provide an additional mechanism of action (LeVine et al., 2009). Here we investigated the effect of these compounds on oligomerization of Aβ under metal-free conditions. The in vivo effects of CQ and PBT2 were also explored on a Caenorhabditis elegans model of Aβ toxicity, showing that hydroxyquinoline treatment results in a change in the in vivo oligomeric status of Aβ.
Materials and Methods
Human and mouse Aβ1–42 was synthesized by the W. M. Keck Laboratory (Yale University, New Haven, CT) and its purity confirmed by high performance liquid chromatography (HPLC) and mass spectrometry, which indicated no significant oxidation or modification. N-terminally fluorescein-tagged Aβ was synthesized in-house using standard Fmoc chemistry. Peptide identity and purity were confirmed by reverse-phase HPLC and mass spectrometry. PBT2 was kindly donated by Prana Biotechnology. CQ was purchased from Sigma (catalog #33931-100MG-R). Solutions of both 8-HQs, PBS, Tris-HCl buffered saline, and water used for buffer preparation were confirmed to be metal free (≪0.03 μm), with inductively coupled plasma mass spectrometry (ICPMS). All other reagents were of analytical grade.
Aβ1–42 preparation.
Aβ1–42 was resupended using a sodium hydroxide-based protocol as described previously (Ryan et al., 2013a). Briefly, 1 mg of peptide was resuspended in 200 μl of 60 mm NaOH and incubated for 5 min at room temperature. This solution was diluted with 700 μl of distilled water and bath sonicated at room temperature for a further 5 min. The sonicated solution was neutralized with 100 μl of 10× PBS, pH 7 (50 mm sodium phosphate, 150 mm sodium chloride) and centrifuged at 14,000 × g. The optical density at 214 nm of the supernatant, containing the resolubilized Aβ1–42, was determined with a quartz microplate and a Flexstation 3 plate reader (Molecular Devices) equipped with absorbance optics. The concentration was calculated from the absorbance value at 214 nm using an extinction coefficient for Aβ1–42 of 95,452 M−1 cm−1 (McColl et al., 2009). Peptide purity was determined using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS; Bruker). Recovery of Aβ1–42 was typically 60%–80% based on dry weight of the peptide.
Thioflavin T (ThT) assays.
The effect of PBT2 on the aggregation of Aβ1–42 was measured using a continuous ThT fluorescence assay described previously (McColl et al., 2009; Ryan et al., 2012, 2013a). PBT2/CQ was prepared as a 1 mm stock in DMSO, and diluted into PBS containing ThT (10 μm) to a final concentration of 10 μm. Aβ1–42 was added to a final concentration of 5 μm, and the plate was incubated at 37°C, with shaking every 7 min, for 3 s, before the measurement of the ThT fluorescence intensity (444 nm excitation and 485 nm emission), using a Flexstation 3 Plate reader. All measurements were conducted in triplicate and averaged, and the experiment was repeated 4 times independently, with different reagent batches. Controls where DMSO alone was added were also conducted.
In addition, point-based-ThT measures were acquired to determine 50% inhibitory concentrations (IC50 values) and the effect of the 8-HQ on preformed fibrils. To determine IC50 values, a series of solutions (in triplicate) were prepared in 1.5 ml microcentrifuge tubes comprising 5 μm Aβ in PBS, pH 7.4, supplemented with 0, 0.8, 1.6, 3.25, 7.5, 10, or 15 μm of PBT2/CQ, and 10 μm ThT. These solutions were incubated at 37°C with shaking. After 24 h, 100 μl aliquots were transferred to 96 well plates and the ThT fluorescence intensity (444 nm excitation, 485 nm emission) was measured in triplicate with the Flexstation 3 plate reader.
To investigate the effect of 8-HQs on preformed fibrils, Aβ peptides (5 μm) were incubated in 1 ml of PBS, 10 μm ThT for 24 h at 37°C with shaking. Increased ThT fluorescence, suggesting the presence of fibrils, was confirmed using the above point-based assay. PBT2 and CQ were added to a final concentration of 10 μm, by adding 1 μl of a 1 mm stock solution per 100 μl of sample. Controls, where the equivalent amount of DMSO was added, were also prepared. The solutions were incubated at 37°C with no shaking and periodically measured by the above point-based assay.
Further analysis was conducted by centrifuging 100 μl of Aβ1–42 solutions incubated with the 8-HQs (after 24 h) or solutions where hydroxyquinolines were added to preformed fibrils at 100,000 × g for 30 min, and then quantifying the protein levels of the supernatant and pellet with a BCA assay (Pierce). All measurements were performed in triplicate.
Electron microscopy.
Solutions from the continuous aggregation assays were vortexed to suspend particulate matter, and an aliquot was spotted onto carbon-coated copper grids (ProSciTech). The grids were washed several times with distilled water to remove excess phosphate and then allowed to air dry. The fibrils were negatively stained with 0.5% uranyl acetate for 3 min and then washed several times with water. The samples were analyzed on a Siemens ELMIS-KOP 102 electron microscope. The films were scanned using a flatbed scanner and the resulting image saved as a tiff file. These experiments were conducted in triplicate, from three different experiments. Twenty images were taken across all grids to ensure that the selected images were representative of the sample.
Analytical ultracentrifugation experiments.
Sedimentation experiments were conducted at a concentration of 15 μm Aβ1–42 using an XL-I analytical ultracentrifuge (Beckman Coulter). Sedimentation velocity measurements were performed at 50,000 rpm and 20°C, using centrifuge cells equipped with quartz windows and charcoal epon dual-sector centerpieces and a sample volume of 300 μl. The sedimentation velocity of the 8-HQ compounds at various ratios with Aβ1–42, solubilized and prepared as described above using PBS as the buffering solution, was investigated by acquiring a radial 350 nm absorbance scan every 7 min.
Sedimentation velocity analysis of Aβ1–42 was measured specifically by using fluorescein-labeled Aβ, which was detected using a Fluorescence Detection System (Aviv Biomedicals), which constitutes a 488 nm excitation laser and detection of fluorescence emission via a photomultiplier tube equipped with a 505 nm long-pass filter. With this system, the fluorescence intensity of 15 μm Aβ1–42 peptide spiked with 1 nm N-terminally fluorescein-labeled Aβ1–42 in the presence and absence of 15 μm 8-HQ was acquired as a function of radial position every 2 min for the duration of the experiment. All velocity data were analyzed using a continuous sedimentation coefficient distribution model [c(S)] and the program SEDFIT version 9.4 (Schuck, 2000, 2003). The amount of sedimenting compound was assessed after baseline correction by taking the average of the first 10 data points of the first scan of the run (total optical density) and subtracting the average of the first 10 points of the final scan (change in optical density). This was expressed as a proportion by dividing the by total optical density and was plotted as function of 8-HQ concentration. The weight average sedimentation coefficient (Sw) was assessed by integrating the c(S) distributions provided by SEDFIT, and this value was also plotted as a function of PBT2 concentration.
Sedimentation equilibrium analysis was also performed. In contrast to the sedimentation velocity, only 100 μl of sample was loaded into the centrifuge cells. Samples were spun at 26,000, 36,000, and 46,000 rpm, and the concentration gradient formed over time at these speeds was monitored using the absorbance at 230 nm. When the samples had reached equilibrium (i.e., there was no change in the concentration gradient over a period of 4 h), a high-quality scan was acquired, using a step size of 10 μm and averaging of 10 separate scans at 230 nm. With this wavelength, there was a significant contribution of the compound absorbance. Data were analyzed using SedpHAT (Vistica et al., 2004) with a model involving two molecular weight (MW) populations, using MW of the hydroxyquinoline (305.5 for CQ, 271.2 for PBT2) as a fixed parameter. This experiment was repeated twice.
Size exclusion chromatography (SEC).
SEC was conducted using an Akta basic FPLC system and a 10 × 300 mm Superdex S75 size exclusion column (GE Healthcare). Samples containing 15 μm Aβ alone, 15 μm PBT2/CQ alone, or mixtures of Aβ and compound were loaded in 1 ml of PBS supplemented with 0.05 mm EDTA. The column was developed at a flow rate of 1 ml per minute using 1× PBS, pH 7.4, 0.05 mm EDTA, and the chromatogram was acquired using a flow absorbance detector set at 280 nm. The column was calibrated using the low MW calibration kit from GE Healthcare containing aprotinin (MW 6.5 kDa), ribonuclease A (MW 13.7 kDa), carbonic anhydrase (MW 29 kDA), ovalbumin (MW 43 kDa), conalbumin (MW 75 kDa), and blue dextran (MW > 2000 kDa).
To measure the stability of the high MW species present in the absence of hydroxyquinolines and the Aβ-dimeric species induced by the presence of 8-HQs, corresponding fractions were collected and reapplied to the SEC column after intervals of 1 and 2 h.
Small angle x-ray scattering (SAXS).
SAXS measurements were acquired at the SAXS/WAXS beamline of the Australian Synchrotron (Kirby et al., 2013). Solutions of Aβ1–42 (1 mg/ml, 221 μm, in PBS) in the presence and absence of 15 μm 8-HQ were analyzed at a camera length of 3.3 m corresponding to a range of momentum transfer 0.005 ≤ q ≤ 0.35 Å−1 (where q = 4πsinθ/λ, 2θ is the scattering angle, and λ is the x-ray wavelength: 1.03 Å at 12 KeV), using a Pilatus 1M detector (Dectris). Data were normalized using an integrated beamstop and intensities put on an absolute scale using distilled water as a standard. To limit radiation damage, 10 frames of 1 s exposure were collected as 50 μl samples/buffers were flowed through a 1.5 mm quartz capillary. The individual frames were compared for agreement before being averaged with the scatterBrain IDL program (http://www.synchrotron.org.au/).
SAXS data were analyzed using the ATSAS software suite (Petoukhov et al., 2007; Petoukhov et al., 2012), including the packages Primus (Konarev et al., 2003) and GNOM, and ab initio models generated using Dammif (Franke and Svergun, 2009). Ensemble optimization modeling was conducted using ensemble optimization method (Bernadó et al., 2007) specifying a dimer of Aβ1–42 for the ensemble generation.
Slot blot retention assay.
A slot blot apparatus (Bio-Rad) was used as per the manufacturer's instructions. A nitrocellulose membrane (0.22 μm) wetted with PBS was used as a filter. Aβ1–42 at a concentration of 5 μm in 100 μl of PBS was mixed with CQ ranging in concentration from 0 to 25 μm and applied to the slot blot apparatus. Each slot blot well was washed thrice with PBS, and then the apparatus was dismantled and the membrane dried at room temperature. The retention assay was performed in triplicate for each sample, and a total of three replicate assays were conducted. The membranes were then imaged using laser ablation (LA)-ICPMS (detailed methods below) or the individual bands of retained material were excised and eluted off of the membrane using 1% nitric acid. The resulting solution was analyzed by bulk ICPMS (detailed methods below). The ability of PBT2 to compete for the CQ binding site was assayed by supplementing the Aβ solution with PBT2 at a constant concentration of 10 μm. The affinity of PBT2 was then assayed by supplementing the Aβ solution with CQ at 10 μm and titrating PBT2 from 0 to 50 μm. All measurements were conducted in triplicate.
LA-ICPMS.
LA-ICPMS analysis and imaging were performed using a NewWave Research UP213 laser ablation unit (Kennelec Scientific), emitting a 213 nm laser pulse in the fifth harmonic. The ablated material was directed to an Agilent 7500ce ICPMS (Mulgrave), fitted with “cs” lenses for enhanced sensitivity. Imaging methods were adapted from those previously described (Austin et al., 2009; Lear et al., 2012). Briefly, a 100 μm laser beam diameter was rastered along the slot blot membrane at 300 μm/s. Iodine (m/z = 127) was monitored in time-resolved analysis mode. Images were produced using purpose-designed ISIDAS imaging software (Hare et al., 2010, 2012).
Bulk ICPMS measurements.
The elemental content of the retained bands was also analyzed by bulk ICPMS measurements using an Agilent 7700x ICPMS (Mulgrave). For typical instrument parameters, refer to Hare et al. (2013) or Rembach et al. (2014). Briefly, the membrane-containing retained material from the slot blot was carefully excised using surgical scissors and covered with 300 μl of 1% nitric acid. The samples were incubated at room temperature for 2 d, and the solution containing the eluted material was carefully aspirated into 1.5 ml Eppendorf tubes. These samples were introduced the ICPMS using the integrated peristaltic pump sample introduction system. Signals from iodine, copper, zinc, and iron were monitored (m/z = 126.9, 63.5, 65.4, and 55.8, respectively).
Binding analysis.
The proportion of 8-HQ that is free and the proportion that is bound to Aβ can be determined from either the retention data, as described above, or from the sedimentation velocity data, also as described above. These data can be relatively easily analyzed by Equation 1, where the proportion of bound hydroxyquinoline per Aβ (R) is given by the maximal proportion of bound hydroxyquinoline (Bmax), the amount of free hydroxyquinoline (Cf), and the dissociation constant (Kd) as follows: This equation was fitted to the titration data using the nonlinear regression tools of Sigmaplot version 12.4, as described previously (Ryan et al., 2011), and converted back to the change in the proportion of sedimenting PBT2 using mass action assumptions.
Analysis of weight average sedimentation coefficient.
Analysis of the change in weight average sedimentation coefficient was conducted in a similar fashion to the methods of Schuck (2003). Briefly, the weight average sedimentation coefficient (Sw) is proportion to the fractional amount of each species that is present in the sedimenting population. The UV absorbance is arising from the hydroxyquinoline alone; thus, the free Aβ, which has no absorbance at 350 nm, is not affecting the weight average sedimentation coefficient we observe. Assuming that the weight average sedimentation coefficient at the highest concentration of PBT2 is equivalent to that of the complex, we can use simple mass action laws to determine the proportion of complex (AC) and free ligand (Cf). Thereby, we can determine the amount of free Aβ (Af) and the dissociation constant (Kd). The two pertinent equations (Eqs. 2 and 3) are as follows: where fAC is the mol fraction of the Aβ/PBT2 complex and SAC is the sedimentation coefficient of the Aβ/PBT2 complex (1.25 S). This equation can be used in conjunction with Equation 3, as follows: to determine the dissociation constant for the complex, as described previously (Schuck, 2003; Bailey et al., 2009; Mok et al., 2011).
NMR analysis.
NMR spectra were acquired at 25°C on a Bruker-BioSpin Avance 700 MHz spectrometer equipped with a cryogenically cooled triple-resonance pulsed field gradient probe. Spectra were obtained on samples that were typically 215 μm Aβ1–42 in 150 mm NaCl, PBS at pH 7.0 in 1H2O:2H2O (95:5). Spectra were referenced to 4,4-dimethyl-4-silapentane-1-sulfonic acid at 0 ppm and processed with TopSpin (Bruker-BioSpin).
Multielectrode array (MEA) measurements of Aβ1–42 toxicity.
For all MEA experiments, we used an MEA2100 system (MultiChannel Systems), with ground plate heating set at 37°C and 60MEA200/30iR-Ti chips (electrode grid: 8 × 8, 60 TiN electrodes; electrode spacing: 200 μm; electrode diameter: 30 μm; MultiChannel Systems). Dissociated primary mouse cortical neurons (E14) were plated (200,000 cells per chip) and grown on poly-l-lysine-coated (1 mg/ml, prepared in borate buffer) MEA chips using standard culture procedures for 2.5 weeks before experimentation. During this time, the cultures were kept in a humidified atmosphere (95% air, 5% CO2, 37°C), where they formed stable neuronal networks. Cells were removed from the incubator and baseline levels of activity recorded for each individual experiment (3 min; using MultiChannel Systems MC Rack version 4.5.13 software). Cells were then replaced in the incubator for ∼30 min before being removed and treated with either Aβ alone (3 μm final concentration on the chip, n = 6), or in combination with CQ (10 μm final concentration on the chip, n = 4). Vehicle controls were performed separately (n = 2) and had no significant effect on activity. Cells were then returned to the incubator for 24 h before a repeated measurement of levels of activity (3 min). For each individual experiment, the number of spikes and bursts were recorded and values post-Aβ and post-Aβ + CQ normalized to the baseline activity for each experiment. Data were then analyzed with GraphPad Prism 6 for Mac OS X (version 6.0b).
Preparation of soluble fraction C. elegans homogenate.
C. elegans strains GMC101, dvIs100 [pCL354(unc-54. DA-Aβ1–42) + pCL26(mtl-2:GFP)], and CL2122; dvIs15(mtl-2:GFP) were cultured on 8P media at 20°C as previously reported (McColl et al., 2012). Embryos were developmentally synchronized via alkaline hypochlorite treatment of gravid adults and overnight hatching of L1 larva in S-basal, 0.1 m NaCl and 0.05 m KxPO3 buffer, pH 6.0 (Brenner, 1974). Approximately 50,000 L1 larvae were transferred to 8P media for 24 h and then an additional 24 h on 8P media ± PBT2 or CQ, with DMSO as the vehicle (final concentration 0.5% v/v). Populations were shifted to 25°C for 24 h to allow the Aβ-induced paralysis phenotype to manifest (McColl et al., 2012). Samples were washed from media with S-basal, and eggs, larvae, and debris were removed via filtration through 40 μm nylon filters (BD Sciences). Approximately 0.5 ml (wet pellet) of adults per replicate was frozen in liquid N2 for lysis. Samples were disrupted in chilled PBS (Invitrogen), with an ice-chilled bath sonicator (Bioruptor, Diagenode), using 5 on–off cycles of 30 s durations on high power (320 W), then clarified by ultracentrifugation (100,000 × g for 30 min at 4°C). Supernatant was removed and kept on ice or immediately used.
Aβ oligomer ELISA assay.
Aβ oligomer levels were determined using the sandwich ELISA with the same antibody used for both capture and detection. The procedures and buffers are similar to those described previously for the monomeric Aβ ELISA (White et al., 2006; Lim et al., 2011). The assay was conducted in 384 well, high-binding polystyrene plates (Greiner). The plate was precoated with 25 μl of 10 μg/ml W02 monoclonal antibody (epitope Aβ5–8) (Ida et al., 1996) in 50 mm sodium carbonate, pH 9.6, at 4°C overnight. After washing with PBST (PBS containing 0.05% Tween), the plate was blocked with 0.5% (w/v) casein in PBS buffer, pH 7.4, to minimize nonspecific binding, and washed with PBS before addition of samples and WO2-biotin (final concentration 0.3 μg/ml, made from a 1 mg/ml stock) and incubated overnight at 4°C. The plate was washed with PBS and europium-labeled streptavidin (final concentration 1 nm, made from a 10 μm stock) added for 1 h at room temperature. After a final wash, the plate was developed with a commercial enhancement solution, which allows the europium to become fluorescent. Time-resolved analysis was performed using a Wallac Victor2 1420 Multilabel Plate Reader (PerkinElmer) with excitation at 340 nm and emission at 613 nm, a delay of 400 μs, and a measurement window of 400 μs. Relative fluorescence levels minus the background were used for each sample as an indication of the Aβ oligomeric levels. Each measurement was performed in triplicate.
Size exclusion analysis of C. elegans homogenates.
The supernatant of C. elegans homogenate (100 μl) was applied to a 10 × 300 mm Superdex S75 size exclusion column (GE Healthcare) using an Agilent 1200 series HPLC. The column was developed at a flow rate of 1 ml per minute using 1× PBS, pH 7.4, 0.05 mm EDTA, and the chromatogram was acquired using a flow absorbance detector set at 280 nm. Fractions (750 μl) were collected across the entire elution, and the amount of monomeric Aβ peptide in each fraction was determined by sandwich ELISA, following the protocol for the oligomer ELISA described above, except that the detection antibody was substituted for 1E8-biotin (epitope 17–22), which, as it has a different detection epitope from the capture antibody (WO2), will detect monomeric, oligomeric, and fibrillar Aβ1–42 peptides.
Results
The effect of PBT2 on Aβ self-association
Hydroxyquinoline effects on Aβ1–42 fibril formation were determined using a continuous ThT assay. Measurements, conducted in triplicate and averaged, indicate that Aβ1–42 alone followed a sigmoidal increase in ThT fluorescence intensity over a 20–24 h time period, whereas Aβ in the presence of CQ or PBT2 did not produce a significant increase in ThT fluorescence (Fig. 1A). This was consistent with our previous studies on inhibitory compounds (Ryan et al., 2012). Inhibition was concentration dependent, with an IC50 of ∼4.7 μm and 5.1 μm for CQ and PBT2, respectively (Fig. 1B). One possibility for this phenomenon is that the hydroxyquinoline competes for ThT-binding sites on Aβ amyloid fibrils. We tested this hypothesis by adding CQ and PBT2 to preformed fibrils simultaneously with an equimolar amount of ThT and monitored the change in ThT fluorescence over time (Fig. 1C,D). Over short time frames (5 min), there was no significant change in ThT fluorescence; however, over longer time frames (24–48 h), there was an ∼50% reduction in the fluorescence intensity of the samples containing CQ or PBT2 compared with that of Aβ alone, which was not significantly changed over the course of the experiment. This was suggestive of longer time processes, such as a disturbance of the equilibria associated with fibril formation toward nonfibrillar species, resulting in a slow redistribution of Aβ1–42 from fibrillar to monomeric nonfibrillar species, rather than competition for binding sites, which would be expected to occur over short time frames. Furthermore, electron micrographs of the time course samples from Figure 1A (representative of three separate time course experiments, Fig. 1E–G) show the presence of fibrillar aggregates only in the Aβ1–42 alone samples; in the Aβ/CQ and Aβ/PBT2 cases, there were no aggregates of any type to be observed. These results are strongly indicative that these 8-HQ do not interfere with ThT binding directly, which is consistent with previous results obtained with a radiolabeled form of CQ that showed that ThT does not compete with 8-HQs for fibril binding sites (Opazo et al., 2006). Further evidence for a loss of amyloid, rather than competition with ThT, was provided by a pelleting assay. The level of protein in the pellet fraction was reduced ∼50% upon treatment with hydroxyquinoline for 24 h, consistent with the changes in the amount of ThT fluorescence (Fig. 1H), indicating a loss of aggregated material as well as a loss of cross β structure.
To further investigate the effect of these 8-HQ on Aβ1–42 self-association, we used SEC under native conditions. Freshly resuspended Aβ was confirmed to exist as an equilibrium mixture of large (>30 kDa) and small (<20 kDa) oligomers (Fig. 2A; 60% high MW, 40% low MW). Application of 1:1 Aβ/8-HQ mixtures to the column resulted in the elution of two major peaks with retention volumes of ∼18.8 and 21 ml, with no evidence of the high MW species. The peak at 21 ml was replicated by conducting the experiment in the presence of PBT2/CQ only (Fig. 2B), indicating that this peak represented the free ligand, whereas the peak at 18.8 ml most likely represented a small, low MW Aβ oligomer. Monomeric Aβ1–42 peptides eluted from this column at ∼19.5 ml.
By fractionating the elution and reapplying the fractionated sample to the column at various time intervals, we investigated the stability of the major oligomers present in the samples. As the major oligomer for Aβ1–42 was centered at ∼10 ml, a 2 ml fraction from 9 to 11 ml was manually collected, and half was reinjected at 1 h, and after 2 h (Fig. 2C). Over this time frame, the peak at 10 min slowly disappeared, and the distribution shifted to resemble the original SEC chromatogram of Aβ1–42 alone. This indicated that all of the oligomers in this sample were in a slow equilibrium and that the high MW species dissociated in the absence of low MW species. Similarly, the 8-HQ-stabilized species at 18 ml was collected manually in a 2 ml fraction from 17 to 19 ml and reapplied to the SEC column (Fig. 2D). Over this time frame, there was a decrease in the peak at 18 ml, and the appearance of both higher and lower MW peaks, consistent with dissociation of the complex resulting in free CQ and Aβ capable of self-associating into higher MW peaks. This dissociation of the stabilized species indicated that the dimer was not stable in the absence of excess hydroxyquinolines, suggesting a low-affinity interaction.
The effects of the 8-HQ on the aggregation state of Aβ were further explored using analytical ultracentrifugation (Fig. 2E–G). Our previous work has shown that low MW Aβ species are formed upon addition of small amphipathic detergent molecules to freshly prepared Aβ, which is itself a heterogeneous mix of small and large oligomers (Ryan et al., 2012). As PBT2 and CQ absorb strongly in the UV range and could potentially bias the analysis, we monitored the oligomeric status of Aβ in solution using trace amounts of fluorescently labeled Aβ peptide. Aβ alone, as has previously been observed (Ryan et al., 2012), displays a main (∼60% of the total) peak at 0.6 S (MW ∼5 kDa) and a distribution of populations with sedimentation coefficients ranging from 5 to 50 S (Fig. 2E; MW ∼21–1000 kDa). Addition of PBT2 or CQ suppressed the formation of large aggregates (sedimentation coefficients 5–50 S) and shifted the low S peak from 0.6 S to 1.2 S (approximate MW of 10–15 kDa) (Fig. 2E). Sedimentation velocity measurements are affected by molecular geometry so a sedimentation equilibrium experiment was conducted to accurately determine the mass of the species. Aβ in the presence of PBT2 (Fig. 2F) or CQ (Fig. 2G) was determined to have a molecular mass of 10.2 ± 0.3 kDa and 9.95 ± 0.26 kDa, respectively. It is not possible to accurately assess the mass of Aβ alone using this method, as equilibrium between sedimentation and diffusion is never reached, presumably because of an equilibrium distribution between aggregated and nonaggregated forms of Aβ that changes over time.
The above results indicate that the presence of equimolar or greater hydroxyquinolines induces the formation of a relatively stable population of low MW species of Aβ. We chose to investigate these species further using Synchrotron SAXS. Aβ in the presence of CQ provided high-quality data for analysis (Fig. 3A), but Aβ in the presence of PBT2 did not provide data of sufficient quality (Fig. 3G) and, thus, was not used in this analysis. Guinier analysis of the CQ:Aβ SAXS data demonstrates that the complex was clearly oligomeric, with an average radius of gyration (Rg) of 35.6 Å (Fig. 3B) [expected Rg for unfolded monomeric Aβ1–42 based on sequence is 18.3 Å (Kohn et al., 2004)], and a Kratky plot representation of the data is consistent with that expected for partially unfolded Aβ (Fig. 3C). The MW of the CQ:Aβ complex estimated from the forward scattering intensity (I0 = 0.010) was ∼11–12 kDa, consistent with the results from analytical ultracentrifugation (AUC) and SEC. The particle distance distribution function, P(r), was calculated via indirect Fourier transformation of the scattering data with Gnom (Svergun, 1992) giving a maximum diameter (Dmax) of 122.45 Å and an average real space Rg of 37.8 Å. De novo reconstruction of the shape of the CQ/Aβ complex was performed using the bead modeling program Dammif (Franke et al., 2009), yielding extended structures with dimensions consistent with dimeric Aβ (Fig. 3D). The orientation of the Aβ1–42 molecules in the dimer cannot be determined by SAXS analysis because of the nonsequence dependence of x-ray scattering.
As the Kratky plot representation and the significantly extended ab initio model suggest a flexible system, an ensemble approach to modeling was subsequently conducted using the ensemble optimization method (Bernadó et al., 2007). This approach generates a pool of conformations (typically 10,000 structures) based on the input protein sequence, and specified oligomerization state (in this case a dimer), and then uses a genetic algorithm to select a subset of structures that best fit the experimental SAXS data. This analysis provides distance distributions (Rg and Dmax) that describe the predominant structural features of the sample in solution. These distributions are shown in Figure 3E, F, where the distributions for the selected structures that fit the SAXS data (fit shown in Fig. 3A, red line) provide relatively narrow distributions relative to the initial pool, with modal values of 34.5 Å (Rg) and 128.6 Å (Dmax). These values are larger than the modal value for the pool distribution indicating that the CQ/Aβ complex is not simply a distribution of random coil dimeric structures, but a more specific extended dimeric structure, and the narrow Rg/Dmax distributions also suggest that the complex is at least partially folded.
Analysis of the interaction of 8-HQ with Aβ
The ThT, pelleting, AUC, SEC, and SAXS results in combination indicate that Aβ in the presence of hydroxyquinoline is most likely a low MW complex, of approximately two 8-HQ (∼700 Da) and two Aβ molecules (9024 Da), giving a stoichiometry of 1 hydroxyquinoline:Aβ molecule. These data, however, do not indicate the affinity of Aβ for 8-HQ. To address this, we initially took advantage of inherent UV absorbance of the 8-HQ molecules, which provide an excellent tool to monitor the association of these compounds with Aβ. Analyzing the absorbance unique to a species in solution, such as PBT2 or CQ, is a powerful tool to measure direct interactions, as exemplified by studies investigating the interaction of 7-nitrobenz-2-oxa-1,3-diazol-4-yl amino-labeled phospholipids with apoC-II (Mok et al., 2011; Ryan et al., 2011), or the interaction of Alexa-488 labeled DNA with Klenow fragment (Bailey et al., 2009). PBT2 and CQ are particularly suited to this approach because they have a low molecular mass and do not sediment effectively in the aqueous-based buffer systems of the experiment. Figure 4 shows the absorbance of 15 μm PBT2 as a function of radial position in the absence of Aβ (Fig. 4A), and PBT2 at 7.5 μm (Fig. 4B) and 22.5 μm in the presence of 15 μm Aβ (Fig. 4C). The addition of Aβ induces the formation of boundaries in the absorbance data, which are dependent on the concentration of PBT2. Similar data for CQ were also acquired and showed a similar trend. This indicates that there is a specific saturable interaction between these two molecules. From the absorbance of PBT2 (or CQ), we can estimate the amount of hydroxyquinoline required to saturate the sedimentation analysis (∼15 μm), which gives a stoichiometry of 1:1 (Fig. 4D). Further analysis of the data at the saturating concentration with the c(S) model in SEDFIT9.4 showed that PBT2 and CQ were associated with an Aβ species having a sedimentation coefficient of 1.25 ± 0.1 S (∼11–14 kDa) (Fig. 4E). Thus, the observed complex has an approximate MW of 10 kDa and consists of two Aβ peptides and two hydroxyquinoline molecules, giving a 2:2, or apparent 1:1, stoichiometry. Analysis of the concentration titration data for both the proportion of sedimenting PBT2 and CQ, and the weight average sedimentation coefficient (obtained by integrating the c(S) distributions, representative data for PBT2 shown in Fig. 4D), indicated an apparent dissociation constant in the low micromolar range (Fig. 4E and Fig. 4F, respectively), which is a relatively weak interaction that appears to have a very significant effect on the self-association of Aβ.
There is a body of research using cell culture and electron paramagetic resonance spectroscopy that suggests Aβ is capable of forming a metal ion-mediated ternary complex with PBT2/CQ (Kenche et al., 2013). The AUC and SEC results were performed with buffers where the metal content is below the detection limit of the methods used and in the presence of an excess of EDTA. Under these conditions, the formation of such a metal ion ternary complex is unlikely; however, categorical, conclusive evidence for the absence of metal ion involvement in the interaction is difficult to address using these assays. Thus, we used ICPMS to determine whether the Aβ/hydroxyquinoline was a ternary complex involving metal ion or a metal independent binary interaction. ICPMS analysis also provides detection that is significantly more sensitive and specific for the compound than the other methods we have used. The approach involved a slot blot membrane retention assay, similar to those used for radioisotope measurements of protein-CQ interactions (Opazo et al., 2006), where the protein (Aβ peptide) is mixed with an increasing concentration of ligand (CQ) and is applied to a nitrocellulose membrane. Instead of using radiation counting to detect the proportion of bound ligand, we used ICPMS to measure the presence of various elements, including iodine (as the unique heteroatom component of CQ), copper, zinc, and iron. For confirmation of our results, we did this analysis via two different sample introduction methods, in two separate locations. First, we used LA-ICPMS to directly measure the elements retained on the nitrocellulose membrane, providing the image in Figure 5A. Second, we used a digestion protocol to solubilize the retained elements, and introduced this solubilized material into the ICPMS with standard liquid handling approaches (solution nebulization ICPMS). The image in Figure 5A shows a clear trend where increasing the concentration of CQ in the Aβ/CQ mixture results in increased retention of iodine, consistent with the formation of a CQ/Aβ complex. This is quantified for LA-ICPMS in Figure 5B, and for bulk ICPMS in Figure 5C, corrected for retention of CQ alone by subtraction of the values shown in Figure 5E, F. Metal ions, including iron, copper, and zinc, showed no significant retention (Fig. 5D). As this is a retention assay and we did not observe any metal present in our hydroxyquinoline, Aβ, or buffer reagents, this indicates that there is no metal retained with the complex. Thus, these metal ions are not significantly associated with the Aβ/CQ complex and, under the conditions used in this study, there is no evidence for the formation of a ternary complex between Aβ, CQ, and a metal ion. Retention of CQ in the absence of Aβ was not significant at the concentrations where CQ is soluble; however, at 50 μm, where CQ is insoluble, we observed appreciable iodine signal (shown in Fig. 5E).
The retained iodine signal is directly proportional to the concentration of the Aβ/CQ complex and consequently provides a measure of the free CQ. These data can be plotted to produce a classical binding curve (Fig. 5D). Nonlinear regression, with a single site binding model, of these data provides a stoichiometry of 1 CQ:1 Aβ and a dissociation constant (Kd) of ∼1 μm (R2 = 0.95 for LA-ICPMS, 0.91 for solution nebulization ICPMS).
Relative affinity of PBT2 and CQ for Aβ
As PBT2 does not contain iodine, we cannot use the above retention assay to directly interrogate the affinity of PBT2 for Aβ. However, we can use competition between the CQ and PBT2 to determine the relative affinity of these compounds for Aβ. Titration of PBT2 against a set concentration of 10 μm CQ in the presence of Aβ resulted in a concentration-dependent decrease in the retained iodine signal, consistent with a classic “cold” competition assay (Fig. 6A). Analysis of the data with a standard one-site competitive binding model provided an approximate equilibrium inhibitor dissociation constant (Ki) of 1.1 μm for the interaction of PBT2 to Aβ. Consistent with the CQ results, there was also no change in the status of any of the metal ions upon titration of PBT2 (Fig. 6B).
Molecular analysis of the Aβ/PBT2 interaction
The observation of apparent hydroxyquinoline:Aβ1–42 complexes with AUC, SEC, and SAXS and an apparent dissociation constant of <10 μm led us to attempt nuclear magnetic resonance analysis of the samples. Using natural isotopic abundance in the peptide sample, concentrations of 1–2 mg/ml (214–428 μm) were required for sufficient sensitivity, and this precludes the use of CQ in these experiments, as its solubility is ∼30 μm in our buffers. PBT2 has no such constraint; thus, these experiments were conducted solely with this 8-HQ. The majority of the Aβ1–42 resonances are unperturbed in the presence of the drug PBT2 (Fig. 7). In the presence of one equivalent of PBT2 to Aβ1–42, resonances associated with PBT2 are significantly broadened with a small chemical shift perturbation (0.04 ppm for the methyl resonances) from the positions of PBT2 in the absence of Aβ and reflect a very low affinity interaction that is in intermediate exchange on the NMR time scale under these conditions (Fig. 7).
Cellular toxicity of the hydroxyquinoline stabilized Aβ1–42 oligomer
To investigate the effect of these Aβ/hydroxyquinoline complexes on neuronal function and toxicity, we conducted an MEA experiment (Fig. 8). This provides information on the effect of a compound or peptide on a neuronal network. We specifically investigated the spikes (Fig. 8A) and bursts (Fig. 8B) of activity in cultured mouse primary cortical neurons after treatment with Aβ alone or Aβ mixed with approximately twofold concentrations of CQ. Aβ alone significantly decreased the activity of the neuronal culture, consistent with previous measures of Aβ toxicity to neuronal cultures. Interestingly, addition of Aβ and CQ not only stopped the reduction in activity but also resulted in a significant increase in activity, consistent with the stabilized Aβ dimer being nontoxic, and CQ enhancing the activity of neurons.
In vivo effects of PBT2 on Aβ1–42 oligomerization in C. elegans
In cell culture, we have shown that the stabilized complex is nontoxic. To investigate the effect of 8-HQs in whole animal models of Aβ toxicity, we used a transgenic C. elegans model that displays paralysis upon expression of Aβ1–42. We previously reported that PBT2 can reduce the toxicity of Aβ1–42 in transgenic C. elegans (McColl et al., 2012). Consistent with the protective effects of 8-HQ, we determined that CQ was also able to significantly reduce toxicity of human Aβ1–42 expressed in the body wall muscle cells (Fig. 9A). Using a range of concentrations, we observed that 24 h exposure to 30 μm PBT2/CQ provided a robust level of protection against Aβ-induced paralysis. This treatment significantly delays the onset of the toxic phenotype but does not completely prevent it. This indicates that these compounds can only partially rescue Aβ toxicity in this model. Initially, we investigated the effect of 8-HQ compounds on Aβ oligomerization using an ELISA, which uses one antibody to both capture and detect Aβ (W02, epitope: Aβ5–8) (Ida et al., 1996), based on the assumption that only oligomeric forms of Aβ will display a second epitope for W0–2 to bind. The validity of this assumption was determined by comparing the effects of equimolar amounts of preaggregated Aβ (which showed a high response in the assay) against freshly prepared and denatured Aβ, both of which showed no response. Soluble lysates of C. elegans treated with PBT2 or CQ had a significant (p < 0.0001), but moderate, 20% reduction in the ELISA signal compared with untreated controls (Fig. 9B). Although this was suggestive of an altered oligomerization state upon treatment, this assay is not quantitative, nor does it provide any indication of the molecular mass of the oligomeric species. Thus, we also examined soluble extracts of C. elegans transgenics using size exclusion fractionation on the Superdex 75 column (Fig. 9C). This analysis shows a clear and significant decrease in high MW Aβ and a small increase in low MW Aβ species (quantified by measuring the area under each respective peak; Fig. 9D), consistent with the AUC and SEC observations described above. Interestingly, the biologically derived Aβ oligomers appear larger than the synthetically derived species. This may be due to increased time to aggregate, a variety of post-translational modifications, or any number of macromolecular interactions that may occur in the complex biological milieu of a whole animal model. Importantly, although treatment with 8-HQ altered the levels of soluble Aβ oligomers in vivo as predicted from the in vitro studies, the treatment did not completely abolish the presence of Aβ oligomers in C. elegans. This result is consistent with both partially reduced phenotype rather than complete prevention of Aβ-induced toxicity (McColl et al., 2012) and with the idea of 8-HQ having multiple modes of action.
Discussion
We have investigated the 8-HQ (CQ) and the novel 8-HQ compound PBT2 for activity in modulating Aβ1–42 self-association and aggregation through a direct interaction. Our aggregation assay results show that these compounds are capable of inhibiting amyloid fibril formation (Fig. 1), and the AUC, SEC, and SAXS results show that both PBT2 and CQ suppress large aggregates (Figs. 2 and 3) and appear to induce a low MW species of Aβ at ∼1:1 stoichiometry (Figs. 2 and 3). The formation of a stable 8-HQ/Aβ complex, however, was not detected by the NMR experiments (Fig. 7). These results parallel other studies on hydroxyquinoline interactions with Aβ, which have shown that hydroxyquinoline-based compounds can inhibit aggregation (LeVine et al., 2009). In contrast to other studies on PBT2 and CQ interacting with Aβ (Kenche et al., 2013), we show that the interaction can be independent of divalent transition elements. Analysis of our buffers and reagents with ICPMS indicates that divalent metal levels are insignificant (≪0.03 μm) and are not retained with the complex on nitrocellulose (Fig. 5). Further support of this conclusion is provided by the size exclusion results, in which the buffers contained 50 μm EDTA and where the same inhibition of aggregation and stabilization of low MW species was observed as in experiments where EDTA was not present.
There are several toxicity studies, including LTP and mouse cognition studies (Cherny et al., 2001; Adlard et al., 2008), cell and yeast culture studies (Abramov et al., 2003; Tardiff et al., 2012; Matlack et al., 2014), and our previous C. elegans experiments (McColl et al., 2012), indicating that Aβ toxicity can be attenuated in the presence of PBT2 or CQ. We have reinvestigated the toxicity of Aβ1–42 in the presence of 8-HQ under our experimental conditions (Fig. 8) and have confirmed that the stabilized dimer is nontoxic. Furthermore, our current C. elegans results confirm a significant change in the toxic phenotype observed with Aβ expression. The previous C. elegans experiments also indicated no significant change in amyloid deposits (McColl et al., 2012), although our current results indicate a significant reduction in oligomeric species. These results are consistent with the current Aβ oligomer hypothesis that oligomeric species of Aβ are toxic and while the amyloid plaques/fibrils are not inherently toxic but a consequence of the aberrant aggregation of Aβ (for review, see Hayden et al., 2013). In particular, our results suggest that high MW (i.e., >20–30 kDa, minimum stoichiometry of a tetramer) oligomers represent the pool of toxic species, whereas low MW oligomers (i.e., dimers) represent a relatively nontoxic form of the peptide. This is consistent with the observation that tetramers may constitute the minimum toxic species (Streltsov et al., 2011). Furthermore, our data support the mechanism that 8-HQ can directly engage Aβ oligomers and monomers to ameliorate the activity of toxic high stoichiometry and MW (>30 kDa) soluble oligomers of Aβ. The previous toxicity (Abramov et al., 2003; Adlard et al., 2008; McColl et al., 2012; Tardiff et al., 2012; Matlack et al., 2014) data in conjunction with our toxicity data (Fig. 8) and characterization of Aβ in the presence of 8-HQ, suggests that these stabilized low MW oligomers are nontoxic. This further indicates that PBT2 and CQ not only stabilize Aβ and suppress aggregation but that these compounds also neutralize large toxic Aβ peptides and oligomers.
In addition to the currently explored mechanisms of action of PBT2 and CQ, we demonstrated that hydroxyquinolines have the capability to directly influence the self-association and in vivo toxicity of the Aβ peptide. This activity is complimentary to the other mechanisms of action described for PBT2, and particularly correlates with the disaggregation of amyloid fibrils, inhibition of reactive oxygen species generated by Aβ copper complexes, and inhibition of dityrosine crosslinks. The stabilization of nontoxic Aβ oligomers does not preclude a metal chaperone activity that can occur in the complex biological milieu (Adlard et al., 2008; Crouch et al., 2011) or an ionophoric activity resulting in promotion of intracellular transition metal uptake by neurons. However, these results do indicate that 8-HQ have an additional beneficial mode of action that relates to the formation and degradation of toxic oligomeric species.
These results also raise some interesting questions in regards to what the toxic oligomeric species may be. Our results suggest that 8-HQ-stabilized low MW (dimeric) Aβ oligomers are not toxic, and the observations in the wider literature of a range of oligomers (for review of Aβ oligomerization, see Hayden et al., 2013) having toxic properties suggest that synaptic loss in AD is mediated by not a single oligomeric species, but a range of different oligomers having varying degrees of toxicity, oligomeric status, and solubility. This complexity may preclude identifying a therapy targeted to a specific oligomer; as such, a targeted approach could miss a multitude of other toxic oligomeric species. An alternative to targeting a specific oligomer for neutralization or depletion is to stabilize a native, nontoxic, or nonpathogenic form of the protein/peptide (for review, see Johnson et al., 2012). This approach has been applied with great success to transthyretin (TTR) systemic amyloidosis, where the natively folded TTR tetramer slowly dissociates, generally under the influence of mutation, to misfolded monomeric proteins that rapidly aggregate via an energetically favorable “downhill” polymerization process into amyloid fibrils (Colon and Kelly, 1992; Hurshman et al., 2004; Foss et al., 2005; Johnson et al., 2005). In this pathway, the rate-limiting step is the dissociation of the native tetramer (Foss et al., 2005), and this has led to a significant effort to identify methods to stabilize the native TTR conformation. This research effort identified Tamafidis [2-(3,5-dichloro-phenyl)-benzoxazole-6-carboxylic acid] for the treatment of TTR systemic amyloidosis in familial amyloid neuropathy (Johnson et al., 2012). This compound acts by binding with high specificity and affinity (∼50 nm) to one of the thyroxine binding sites on TTR and stabilizing the dimer–dimer interface of the tetramer through hydrophobic and electrostatic interactions (Bulawa et al., 2012). This prevents the dissociation of the tetramer and thus formation of misfolded monomer and amyloid fibrils (Bulawa et al., 2012). Tafamidis was shown to significantly slow progression of the disease process, including autonomic and peripheral neuropathies, and improved autonomic nervous system function and cachexia (Coelho et al., 2012, 2013). This efficacy proves the concept that stabilization of a native, stable conformation can have significant outcomes in protein misfolding diseases. Although TTR follows a different pathway from the typical nucleated polymerization (NP) models applied to amyloidogenesis, the activation energy for monomer dissociation is akin to the energy required to form a nucleus in the NP model. This suggests that similar strategies, where a nontoxic conformation is stabilized by the binding of particular compounds, may be applicable to the wider range of amyloid related diseases. In support of this theory, a recent study on the effect of gallic acid interacting with α-synuclein showed that the binding of this compound prevented the collapse of the protein into an aggregation prone form (Liu et al., 2014). Interestingly, this interaction, while having large and significant effects on the biophysics of the protein, also only displayed extremely subtle effects when investigated by NMR (Liu et al., 2014). Our results lend further support to these theories, suggesting that PBT2/CQ variants have an additional mechanism of action whereby Aβ is stabilized in a nontoxic low MW form.
Footnotes
The work was supported by the Australian National Health and Medical Research Council Program Grant 628946 and Project Grant 1050751 (G.M.), the Knott Family Equipment Grant, and the Pierce Armstrong Trust Grant. T.M.R. was supported by the Australian Alzheimer's Dementia Research Foundation full fellowship. The Florey Institute of Neuroscience and Mental Health is supported by Operational Infrastructure Support funding from the Victorian State Government. The authors thank Dr. Robert Cherny and Prana Biotechnology for their comments on our data.
C.L.M., P.A.A., and K.J.B. are advisors to and have interests in Prana Biotechnology. The remaining authors declare no competing financial interests.
- Correspondence should be addressed to Dr. Timothy M. Ryan, Florey Institute of Neuroscience and Mental Health, Kenneth Myer Building, Genetics Lane, University of Melbourne, Parkville 3052, Victoria, Australia. tmryan{at}unimelb.edu.au