Figure 4. Wnt signaling transforms OPC to GPCs by repressing Hth expression. A, Hth (magenta) is not expressed in NBs (Dpn+, blue) in the GPC region (green) in control larvae (white arrows). B, Expressing dominant negative form of Dfz2 under the control of wg-Gal4, Hth (magenta) was ectopically expressed in NBs in GPC region (Dpn+, blue; white arrows). C, Expressing hth under the control of wg-Gal4, Bsh+ (blue, yellow arrows) and Drf+ (magenta, white arrows) neurons were ectopically formed within GPC region compared with the control in Figure 2A. D, Inducing hth expression, ventral Eya+ (magenta) neurons were lost (yellow arrow), and Bsh+ (blue) neurons were ectopically formed (white arrow) compared with the control in Figure 1C. E, Inducing Hth expression under the control of omb-Gal4, Bsh+ (blue, yellow arrows) and Drf+ (magenta, white arrows) neurons were ectopically observed forming complete circles compared with the control in Figure 2C. F, Inducing Hth expression, Eya+ neurons (magenta) were completely lost (yellow arrows) and Bsh+ neurons (blue, white arrow) were ectopically observed compared with the control in Figure 2E. G, H, Dominant negative form of Pan under the control of omb-Gal4 induced ectopic Bsh+ (blue in G) and Drf+ (magenta in G) neurons within GPC region (G, yellow and white arrows, respectively). Eya+ neurons were reduced (magenta in H; white arrows). I, Simultaneously suppressing Hth expression and Wnt signaling by expressing dominant negative form of Pan and Hth RNAi, ectopic Bsh+ (blue) and Drf+ (magenta) neurons were disappeared (white arrows; compare with G). J, The number of Eya+ neurons (magenta) was not rescued (white arrows) by simultaneous suppression of Hth expression and Wnt signaling (compare with H). K, Hth expression was completely suppressed by induction of Hth RNAi under the control of optix-Gal4. L, Hth (magenta) expression in NBs (Dpn+, blue) was suppressed (white arrows) by Hth RNAi and Wnt signaling inhibition. Insets, Magnified images of the areas indicated by yellow squares. All of the images, except B, are at the same magnification shown by a scale bar in A.