Article Information
- Received July 28, 2015
- Revision received December 27, 2015
- Accepted January 18, 2016
- First published March 2, 2016.
- Version of record published March 2, 2016.
Author Information
Author contributions
Author contributions: E.G., S.B., and F.R. designed research; E.G. and S.B. performed research; S.B. and F.R. contributed unpublished reagents/analytic tools; E.G., S.B., and F.R. analyzed data; E.G. and F.R. wrote the paper.
Disclosures
- Received July 28, 2015.
- Revision received December 27, 2015.
- Accepted January 18, 2016.
This work was supported by the CNRS, ENS-Lyon, University of Lyon, and Agence Nationale de la Recherche Grant 10BLAN121901. E.G. received a Ministère de l'Enseignement Supérieur et de la Recherche fellowship. We thank Andreas Faissner for C-6-S antibodies; Estelle Hirsinger for the eng2a probe; Stone Elworthy and Fredericus van Eeden for dnPKA, Shh, and smyhc1 constructs and ptch1/ptch2 mutant embryos; Michael Granato for unplugged/MuSK embryos; Magali Naville (Institut de Génomique Fonctionnelle de Lyon, Lyon, France) for bioinformatic analysis; Laure Bernard and Robert Renard (Plateau de Recherche Expérimentale de Criblage In vivo; PRECI) for fish care and the PLATIM (SFR Lyon-Gerland Biosciences, France); Marilyne Malbouyres and Elisabeth Vaganay for expert technical assistance; Jamilé Hazan for sharing protocols and advice; and Dominique Le Guellec for his continuing interest in the study.
The authors declare no competing financial interests.
- Correspondence should be addressed to Dr. Florence Ruggiero, Institut de Génomique Fonctionnelle de Lyon, UMR CNRS 5242, ENS de Lyon, 46, Allée d'Italie, F-69364 Lyon Cedex 07, France; florence.ruggiero{at}ens-lyon.fr
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