Figure 5. GluN2B S1413L cannot rescue NMDA EPSCs. A, Loss of evoked NMDA EPSCs in Cre-positive GRIN2Afl/flGRIN2Bfl/fl hippocampal CA1 neurons at P13–P15. Amplitude: Control (Cnt), 71.9 ± 9.9 pA; Cre, 4.2 ± 0.9 pA, n = 10. ***p < 0.0001. B, Coexpression of Cre-mCherry with GluN2B-IRES-GFP rescued NMDA EPSCs Amplitude: Cnt, 71.8 ± 12.2 pA; Cre+GluN2B, 71.9 ± 8.8 pA, n = 16. p = 0.99. C, Coexpression of Cre-mCherry with GluN2B S1413L–IRES-GFP did not fully rescue NMDA EPSCs [103 ± 8.5 pA; Cre + GluN2B(S1413L), 66 ± 7.9 pA]. n = 16. **p = 0.0042. D, GluN2B S1413L variant does not change the NMDA EPSC decay time constant compared with GluN2B. Averaged and peak-aligned NMDA EPSCs were recorded from control or transfected cells from GRIN2Afl/flGRIN2Bfl/fl mice [decay time constant: Cnt, 220.0 ± 9.4 ms, n = 32; Cre + GluN2B, 265.9 ± 16.4 ms, n = 16; Cre + GluN2B(S1413L), 268.2 ± 11.3, n = 16. Cnt vs Cre + GluN2B, p = 0.012 < 0.05; Cnt vs Cre + GluN2B(S1413L), p = 0.0033 < 0.01; Cre + GluN2B vs Cre + GluN2B(S1413L), p = 0.91]. Scale bar, 50 pA and 0.02 s. Error bars indicate SEM.