Figure 3. A, Taste-evoked imaging responses in layer II/III GC neurons. Fluorescence traces show that multiple taste presentations separated by several minutes evoke similar responses from the three labeled neurons. Horizontal scale bar, 5 s; vertical scale bar, 200% ΔF/F. B, Individual cell response thresholds were set at 2.0, 2.5, 3.0, and 3.5 SDs and the percentage of cells responding to only one, two, three, or four tastants were compared. All other thresholds (grays) produced highly similar percentages of cells as the 2.5 (red) chosen for analysis. C, Example traces from four different GC neurons to taste application (black bar). D, Normalized taste-evoked responses from all cells taken from the field shown in Figure 2B. White line depicts onset of taste delivery. Each row in the “heat map” represents a cell and time is represented along the x-axis. Individual neuron responses vary in their selectivity and temporal response. E, All responsive neurons grouped into best-stimulus categories (colors) and arranged in descending order of response magnitude to that stimulus. F, Percentage of cells by best taste stimulus. G, Similar percentages of cells significantly responding to each taste. H, Mean fluorescence change evoked by each taste across the cell population.