Figure 3. Neuronal loss and reactive astrocytes in germline-KI and CaMKIIα-KI mice. A, B, Western blotting analysis of NeuN and GFAP levels in the cortex (A) and calbindin and GFAP levels in the cerebellum (B) of 3-month-old germline-KI and CaMKIIα-KI mice. 1C2 was used to indicate the expression of mutant TBP. Vinculin was used as loading controls. The ratios of NeuN, calbindin, or GFAP to vinculin on Western blots are presented beneath the blots (one-way ANOVA, n = 3). Arrows indicate mutant TBP. C, Immunohistochemistry staining of NeuN and GFAP in the cortex of germline-KI and CaMKIIα-KI mice. 1C2 staining was used to show the expression of mutant TBP (scale bars, 20 μm). D, Immunohistochemistry staining of calbindin and GFAP in the cerebellum of germline-KI and CaMKIIα-KI mice (scale bars: for calbindin, 50 μm; for GFAP and 1C2, 20 μm). E, Quantitative analysis of NeuN, calbindin, and GFAP staining intensity in D (one way ANOVA, n = 5). F, G, Nissl staining (F) and quantification (G) of neurons in the cortex of WT, CaMKIIα-KI, and germline-KI mice (scale bar, 20 μm; one-way ANOVA, n = 5). Data are represented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.