In the article, “A Role for RhoB in Synaptic Plasticity and the Regulation of Neuronal Morphology” by Kara McNair, Rosemar Spike, Clare Guilding, George C. Prendergast, Trevor W. Stone, Stuart R. Cobb, and Brian J. Morris, which appeared on pages 3508–3517 of the March 3, 2010 issue, the authors have become aware of an error in Figure 4, where bands from a total cofilin immunoblot (Ai) were inadvertently also pictured as total Lim kinase bands (Bi). We have investigated this error, and believe that it derived from a file labeling error on the discs used to transfer images between computers. We have reanalyzed scans of original phospho-cofilin, total cofilin, phospho-LIMK, and total LIMK films. A revised Figure 4 now shows the correct images. A non-specific band from the total LIMK film is now also shown as a further loading control. In addition, we have re-quantified the data from the scans of the original films, and obtain equivalent results to those shown in Figure 4, Aii and Bii. Scans of the full film images are now also included as supplementary data to the article. The authors apologize for the error, but affirm that the description and interpretation of the data reported in the article are not affected. The corrected Figure 4 and amended legend are now shown in the online PDF version and displayed below.
Figure 4. Western blot analysis of cofilin and LIMK phosphorylation in the CA1 hippocampal region from RhoB+/+ and RhoB−/− mice, following tetanus-induced LTP. Ai, Western blot images illustrating levels of pCofilin in isolated CA1 regions of hippocampal slices previously subjected to HFS and 15 min synaptic potentiation. Bands from RhoB+/+ (+/+) animals show an increase in intensity following LTP induction. No change in band intensity is seen in tissue from RhoB−/− (−/−) slices after LTP induction. Levels of total cofilin remained constant throughout the time course of the experiment. Aii, In acute RhoB+/+ hippocampal slices, levels of phosphorylated cofilin were significantly increased 15 min following the delivery of a single high-frequency stimulation to the Schaffer collaterals of CA1 pyramidal cells. In RhoB−/− animals, no increase in cofilin activation was observed following induction of LTP. Data are expressed as mean ± SEM. Bi, Western blot images illustrating levels of pLIMK in isolated CA1 regions of hippocampal slices treated as in A. A non-specific band is also shown as an additional loading control. Bii, Levels of phosphorylated LIMK showed a tendency to increase in RhoB+/+ tissue following LTP induction, while pLIMK levels were significantly reduced in tissue from RhoB−/− mice. n = 7–8; *p < 0.05 vs RhoB+/+ control: two-way ANOVA followed by Tukeys post hoc analysis. Numbers (Ai, Bi) represent size markers (kilodaltons).