Figure 1. Excitatory PBN and BLA projections to CeA. A, A diagram illustrating bilateral delivery of a viral vector into the PBN in rats. Red dots indicate PBN. B, C, Representative immunohistochemical images of ChR2-mCherry expression in PBN (B) and in the CeA (C), but not in the BLA, 10 d after bilateral infusion of the viral vector AAV5-CaMKIIα-ChR2-mCherry into PBN of a rat. D, CeA image in higher-magnification for mCherry-expressing terminals (red) and DAPI-stained cells (blue). E, Image of ChR2-GFP expression in BLA and CeA 10 d after bilateral infusion of the viral vector AAV5-CaMKIIα-ChR2-GFP into BLA of a rat. F, CeA image in higher-magnification for GFP-expressing terminals (green) and DAPI-stained cells (blue). Scale bars: B, C, 500 μm; D, 100 μm; E, 200 μm; and F, 20 μm.