Figure 2. RhebCA expression induces S6 phosphorylation and somatic hypertrophy in a dose-dependent manner. A, Diagram of a coronal brain section illustrating the electroporated region (green) of the cortex. Ipsilateral side is the electroporated cortex; contralateral side is the non-electroporated cortex. Red square indicates the area imaged in Figure 2B. Ipsi, Ipsilateral; Contra, contralateral; ctx, cortex; cc, corpus callosum; ml, midline. B, Inverted grayscale images of p-S6 staining in ipsilateral and contralateral cortices from control and low, intermediate, and high RhebCA mice. Scale bar, 200 μm. C, Quantification of p-S6 staining intensity in ipsilateral cortices. p < 0.0001 by one-way ANOVA. Tukey's post hoc test: *p < 0.05, **p < 0.01, ****p < 0.0001. D, Images of p-S6 staining (red) and GFP+ cells (green) in ipsilateral cortices from control and low, intermediate, and high RhebCA mice. Scale bar, 100 μm. E, Quantification of p-S6 staining intensity in GFP+ (electroporated) cells. p < 0.0001 by one-way ANOVA. Tukey's post hoc test: *p < 0.05, **p < 0.01, ****p < 0.0001. F, Scatterplot of cellular versus cortical p-S6 staining intensity. r = 0.8501, p < 0.0001 by Pearson's product–moment correlation. G, Images of GFP+ cells from control and low, intermediate, and high RhebCA mice. White squares on the top panel are enlarged on the bottom panel. Scale bar, 50 μm (top and bottom). H, Quantification of GFP+ cell soma size. p < 0.0001 by one-way ANOVA. Tukey's post hoc test: ****p < 0.0001. I, Scatterplot of GFP+ cell soma size versus cortical p-S6 staining intensity. r = 0.8257, p < 0.0001 by Pearson's product–moment correlation. Error bars indicate ± SEM. Con, Control; Int, intermediate.