Disruption of RAB-5 increases EFF-1 fusogen availability at the cell surface and promotes the regenerative axonal fusion capacity of the neuron

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¹Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane QLD 4072, Australia.
²Neuroscience Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia.

Correspondence to: m.hilliard{at}uq.edu.au (M.A.H.), r.giordanosantini{at}uq.edu.au (R.G.S.)

Author contributions: C.L., B.N., R.G.-S., and M.A.H. designed research; C.L., M.A.R., X.Y.H., and R.G.-S. performed research; C.L., B.N., R.G.-S., and M.A.H. analyzed data; C.L. wrote the first draft of the paper; C.L., B.N., R.G.-S., and M.A.H. edited the paper; M.A.H. wrote the paper.

The authors declare no competing financial interests.
We thank Luke Hammond and Rumelo Amor for help with microscopy, as well as Rowan Tweedale and members of the Hilliard lab for helpful discussion and comments. We also thank Guangshuo Ou for the pOG172 plasmid, Nick Valmas for the PNV MYR::mCherry plasmid, and Elia Di Schiavi for advice regarding cell-specific RNAi. This research was supported by a University of Queensland Research Scholarship to C.L. and X.Y.H., a University of Queensland Development Fellowship to M.A.R., and a Human Frontier Science Program Fellowship LT000762/2012 to R.G.S. Some strains were provided by the Caenorhabditis Genetic Center (CGC), which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). This work was supported by NHMRC project grants (1068871 and 1129367), an ARC discovery project (160104359) and an NHMRC Senior Research Fellowship (1111042) to M.A.H.; NHMRC project grants (1101974 and 1099690) to B.N. The LSM 710 META confocal microscope used in this study was supported by an ARC Grant (LIEF LE130100078).