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Cover picture: Periventricular tyrosine hydroxylase (TH)-containing neurons in neonatal rat midhypothalamic tissue in vivo and in vitro. Left, Coronal in vivo view of tissue taken for culturing at the level of the paraventricular nucleus immunostained for TH and counterstained with methyl green (bright-field photomicrograph). Dark brown cells are dopaminergic neurons. The third ventricle is right, and ventral is bottom. Right, Slice explant at the level of the paraventricular nucleus maintained for 18 d in culture and processed for TH mRNA in situ hybridization histochemistry using a synthetic, radiolabeled deoxynucleotide antisense probe. Dopaminergic neurons are visualized by the clustering of silver grains over cells (dark-field photomicrograph). In the explant culture, the third ventricle is left and ventral is bottom. Note that the distribution of TH mRNA expressing neurons in slice culture mirror that of the TH-immunostained neurons in vivo. Organotypic cultures were used to calculate TH mRNA turnover in discrete dopaminergic populations within the hypothalamus. For details, see the article by Maurer and Wray in this issue (pp. 4552-4561).
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