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Cover picture: Confocal laser-scanning microscope
compressed series of optical sections through the auditory sensory epithelium (basilar papilla) from the cochlea of a chick treated with
three 100 µg · ml
1 · d
1 injections of
gentamicin, examined at 4 d after the onset of injections.
Yellow-green fluorescence shows anti-synapsin Ia/IIa reactivity; red fluorescence results from binding of
rhodamine-conjugated phalloidin to polymerized actin. In this
transition zone between the area of complete hair cell loss at the
proximal end and the distal part of the cochlea where hair cells are
not affected, the tall hair cells are still present in the superior
side of the cochlea (note the rhodamine-labeled stereocilia bundles to the right). These are associated with bouton-type efferent
terminals, which label with the antibody against synapsin. Hair cells
in the inferior part of the cochlea have been lost, and the large cup-shaped efferent terminals that innervate them have broken apart,
leaving "blobs" of synapsin reactivity in the basilar papilla as
shown. For details, see the article by Hennig and Cotanche, in this
issue (pages 3282-3296).
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