Cover picture: Immunohistochemical localization of GABA
and
7 nAChR expression in cultured hippocampal neurons.
Phase-contrast cells dissociated from P5 rat hippocampus and cultured
for 8 days in vitro are shown. Anti-GABA antibody
(green) labeled the large neuron but not the smaller
neurons. An antibody against the
7 subunit of nAChR (red)
labeled the large neuron and one small neuron. The anti-GABA image and
anti-
7 image were superimposed, and the areas of overlap appear
yellow. Inset, Enlarged views of the boxed
area. A synaptic localization of
7 subunits is suggested by the
hotspots of
7 subunits on GABAergic neurites. For details, see the
article by Liu et al. in this issue (pages 5660-5669).