RT Journal Article SR Electronic T1 LRRK2 Controls Synaptic Vesicle Storage and Mobilization within the Recycling Pool JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 2225 OP 2237 DO 10.1523/JNEUROSCI.3730-10.2011 VO 31 IS 6 A1 Giovanni Piccoli A1 Steven B. Condliffe A1 Matthias Bauer A1 Florian Giesert A1 Karsten Boldt A1 Silvia De Astis A1 Andrea Meixner A1 Hakan Sarioglu A1 Daniela M. Vogt-Weisenhorn A1 Wolfgang Wurst A1 Christian Johannes Gloeckner A1 Michela Matteoli A1 Carlo Sala A1 Marius Ueffing YR 2011 UL http://www.jneurosci.org/content/31/6/2225.abstract AB Mutations in leucine-rich repeat kinase 2 (LRRK2) are the single most common cause of inherited Parkinson's disease. Little is known about its involvement in the pathogenesis of Parkinson's disease mainly because of the lack of knowledge about the physiological role of LRRK2. To determine the function of LRRK2, we studied the impact of short hairpin RNA-mediated silencing of LRRK2 expression in cortical neurons. Paired recording indicated that LRRK2 silencing affects evoked postsynaptic currents. Furthermore, LRRK2 silencing induces at the presynaptic site a redistribution of vesicles within the bouton, altered recycling dynamics, and increased vesicle kinetics. Accordingly, LRRK2 protein is present in the synaptosomal compartment of cortical neurons in which it interacts with several proteins involved in vesicular recycling. Our results suggest that LRRK2 modulates synaptic vesicle trafficking and distribution in neurons and in consequence participates in regulating the dynamics between vesicle pools inside the presynaptic bouton.