PT  - JOURNAL ARTICLE
AU  - Man Tang
AU  - Kenneth A. Pelkey
AU  - David Ng
AU  - Evgueni Ivakine
AU  - Chris J. McBain
AU  - Michael W. Salter
AU  - Roderick R. McInnes
TI  - Neto1 Is an Auxiliary Subunit of Native Synaptic Kainate Receptors
AID  - 10.1523/JNEUROSCI.6617-10.2011
DP  - 2011 Jul 06
TA  - The Journal of Neuroscience
PG  - 10009--10018
VI  - 31
IP  - 27
4099  - http://www.jneurosci.org/content/31/27/10009.short
4100  - http://www.jneurosci.org/content/31/27/10009.full
SO  - J. Neurosci.2011 Jul 06; 31
AB  - Ionotropic glutamate receptors of AMPA, NMDA, and kainate receptor (KAR) subtypes mediate fast excitatory synaptic transmission in the vertebrate CNS. Auxiliary proteins have been identified for AMPA and NMDA receptor complexes, but little is known about KAR complex proteins. We previously identified the CUB (complement C1r/C1s, Uegf, Bmpl) domain protein, Neto1, as an NMDA receptor-associated polypeptide. Here, we show that Neto1 is also an auxiliary subunit for endogenous synaptic KARs. We found that Neto1 and KARs coimmunoprecipitated from brain lysates, from postsynaptic densities (PSDs) and, in a manner dependent on Neto1 CUB domains, when coexpressed in heterologous cells. In Neto1-null mice, there was an ∼50% reduction in the abundance of GluK2-KARs in hippocampal PSDs. Neto1 strongly localized to CA3 stratum lucidum, and loss of Neto1 resulted in a selective deficit in KAR-mediated neurotransmission at mossy fiber-CA3 pyramidal cell (MF-CA3) synapses: KAR-mediated EPSCs in Neto1-null mice were reduced in amplitude and decayed more rapidly than did those in wild-type mice. In contrast, the loss of Neto2, which also localizes to stratum lucidum and interacts with KARs, had no effect on KAR synaptic abundance or MF-CA3 transmission. Indeed, MF-CA3 KAR deficits in Neto1/Neto2-double-null mutant mice were indistinguishable from Neto1 single-null mice. Thus, our findings establish Neto1 as an auxiliary protein required for synaptic function of KARs. The ability of Neto1 to regulate both NMDARs and KARs reveals a unique dual role in controlling synaptic transmission by serving as an auxiliary protein for these two classes of ionotropic glutamate receptors in a synapse-specific fashion.