RT Journal Article
SR Electronic
T1 Induction of the myelin proteolipid protein (PLP) gene in C6 glioblastoma cells: functional analysis of the PLP promotor
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 3060
OP 3069
DO 10.1523/JNEUROSCI.11-10-03060.1991
VO 11
IS 10
A1 KA Nave
A1 G Lemke
YR 1991
UL http://www.jneurosci.org/content/11/10/3060.abstract
AB The terminal differentiation of postmitotic oligodendrocytes is marked by the induction of myelin-specific genes. In this report, we demonstrate that culture conditions that induce oligodendrocyte differentiation of glial progenitor cells also induce differentiation of C6 glioblastoma cells, as monitored by activated transcription of the gene encoding proteolipid protein (PLP), the major myelin protein of the CNS. When assayed by transfections of hybrid reporter plasmids, the transcriptional control region of the PLP gene is preferentially active in differentiated C6 cells and contains both positive and negative cis-regulatory elements. In general, functional identification of these elements is well correlated with the binding sites of glial nuclear proteins, as visualized by the presence of DNase I-protected footprints. A sequence within one positive cis-regulatory element of the PLP gene is conserved in the control regions of three other myelin- specific genes, suggesting that their coordinate transcription may involve a common regulatory mechanism.