RT Journal Article
SR Electronic
T1 Immunofluorescent localization of three Na,K-ATPase isozymes in the rat central nervous system: both neurons and glia can express more than one Na,K-ATPase
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 381
OP 391
DO 10.1523/JNEUROSCI.11-02-00381.1991
VO 11
IS 2
A1 KM McGrail
A1 JM Phillips
A1 KJ Sweadner
YR 1991
UL http://www.jneurosci.org/content/11/2/381.abstract
AB In the CNS, there are multiple isozymes of the sodium and potassium ion- stimulated adenosine triphosphatase (Na,K-ATPase) that have differences in affinity for Na+, ATP, and cardiac glycosides. Three forms of the catalytic subunit (designated alpha 1, alpha 2, and alpha 3) are known to be derived from different genes, but little is known of the cellular distributions of the proteins or their physiological roles. Isozyme- specific monoclonal antibodies permitted the immunofluorescent localization of the 3 Na,K-ATPases in the rat CNS, and markedly different patterns of staining were seen. All 3 isozymes were detected, singly or in combination, in 1 or more neuronal structures, while both alpha 1 and alpha 2 were detected in glia. Many different neuroanatomic structures or cell types stained for more than 1 isozyme. Even when a structure or region stained for more than 1 isozyme, the pattern of staining was frequently dissimilar, suggesting complex differences in gene expression and cellular localization.