TY - JOUR T1 - Cyclic AMP-dependent phosphorylation of the rat brain ryanodine receptor JF - The Journal of Neuroscience JO - J. Neurosci. SP - 1094 LP - 1100 DO - 10.1523/JNEUROSCI.12-03-01094.1992 VL - 12 IS - 3 AU - A Yoshida AU - A Ogura AU - T Imagawa AU - M Shigekawa AU - M Takahashi Y1 - 1992/03/01 UR - http://www.jneurosci.org/content/12/3/1094.abstract N2 - Phosphorylation of the rat brain ryanodine receptor was studied using a monoclonal antibody, Ry-1, against the cardiac ryanodine receptor. A large polypeptide with the same SDS-PAGE mobility as that of the canine cardiac receptor was detected in rat brain membranes by immunoblotting. The brain ryanodine receptor was solubilized from the microsomal membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), and more than 85% of the solubilized receptor was immunoprecipitated by Ry-1. Immunoprecipitated receptors were phosphorylated by cAMP-dependent protein kinase. The ryanodine receptor was also expressed in cultured fetal rat brain neurons and was phosphorylated by treating the cells with dibutyryl cAMP. The number of cells showing a caffeine-induced Ca2+ transient was increased significantly in the phosphorylating condition. These results suggest that the Ca channel activity of the brain ryanodine receptor is regulated by cAMP-dependent phosphorylation. ER -