RT Journal Article
SR Electronic
T1 Cyclic AMP-dependent phosphorylation of the rat brain ryanodine receptor
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 1094
OP 1100
DO 10.1523/JNEUROSCI.12-03-01094.1992
VO 12
IS 3
A1 A Yoshida
A1 A Ogura
A1 T Imagawa
A1 M Shigekawa
A1 M Takahashi
YR 1992
UL http://www.jneurosci.org/content/12/3/1094.abstract
AB Phosphorylation of the rat brain ryanodine receptor was studied using a monoclonal antibody, Ry-1, against the cardiac ryanodine receptor. A large polypeptide with the same SDS-PAGE mobility as that of the canine cardiac receptor was detected in rat brain membranes by immunoblotting. The brain ryanodine receptor was solubilized from the microsomal membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), and more than 85% of the solubilized receptor was immunoprecipitated by Ry-1. Immunoprecipitated receptors were phosphorylated by cAMP-dependent protein kinase. The ryanodine receptor was also expressed in cultured fetal rat brain neurons and was phosphorylated by treating the cells with dibutyryl cAMP. The number of cells showing a caffeine-induced Ca2+ transient was increased significantly in the phosphorylating condition. These results suggest that the Ca channel activity of the brain ryanodine receptor is regulated by cAMP-dependent phosphorylation.