PT  - JOURNAL ARTICLE
AU  - FF Borisy
AU  - GV Ronnett
AU  - AM Cunningham
AU  - D Juilfs
AU  - J Beavo
AU  - SH Snyder
TI  - Calcium/calmodulin-activated phosphodiesterase expressed in olfactory receptor neurons
AID  - 10.1523/JNEUROSCI.12-03-00915.1992
DP  - 1992 Mar 01
TA  - The Journal of Neuroscience
PG  - 915--923
VI  - 12
IP  - 3
4099  - http://www.jneurosci.org/content/12/3/915.short
4100  - http://www.jneurosci.org/content/12/3/915.full
SO  - J. Neurosci.1992 Mar 01; 12
AB  - We show that calmodulin-dependent phosphodiesterase (CAM-PDE) is selectively expressed in mature olfactory receptor neurons within the olfactory mucosa. Immunocytochemical staining reveals neuronal immunoreactivity that is most pronounced within cilia, dendritic knobs, and axon bundles. Neither sustentacular cells nor basal cells display immunoreactivity. The extent of loss of neuronal immunoreactivity following bulbectomy resembles loss of the neuronal population. High- affinity CAM-PDE activity in olfactory cilia is fivefold greater than in brain, when assayed at low micromolar cAMP. This activity is depleted in turbinates following bulbectomy. Olfactory mucosal PDE activity is composed of a minimum of two major forms. In the absence of Ca(2+), rolipram-sensitive PDE comprises 65% of total activity. Following stimulation by Ca2+, CAM-PDE activity is elevated sixfold to become the predominant form, thereby increasing total activity 300%, with half-maximal effect at 1 microM Ca2+. We propose that Ca2+ stimulation of CAM-PDE may be necessary for termination of olfactory signals.