TY - JOUR T1 - Specific high-affinity receptors for neurotrophin-3 on sympathetic neurons JF - The Journal of Neuroscience JO - J. Neurosci. SP - 2610 LP - 2616 DO - 10.1523/JNEUROSCI.13-06-02610.1993 VL - 13 IS - 6 AU - G Dechant AU - A Rodriguez-Tebar AU - R Kolbeck AU - YA Barde Y1 - 1993/06/01 UR - http://www.jneurosci.org/content/13/6/2610.abstract N2 - When used at concentrations allowing interactions only with its high- affinity receptors, neurotrophin-3 (NT-3) promotes the survival of sensory neurons isolated from embryonic day 8 (E8) chicks, but not the survival of E11 sympathetic neurons. These sympathetic neurons (which can be rescued by the addition of NGF) display high-affinity receptors for NT-3 (Kd of 1.6 x 10(-11) M) that cannot be distinguished from the high-affinity NT-3 receptors on sensory neurons using equilibrium binding or kinetic criteria. This represents the first example of embryonic neurons that cannot be rescued by the in vitro addition of a neurotrophin in spite of the presence of corresponding neurotrophin high-affinity receptors. At elevated concentrations, beyond the saturation of its high-affinity receptors, NT-3 supports the survival of some E11 sympathetic neurons, an effect that might be mediated by the high-affinity NGF receptor. Using E7 sympathetic neurons, about 40% of the cells initially plated can be rescued in vitro by the addition of low concentrations of NT-3 (but not of NGF) and produce profuse neurites. This indicates that NT-3 may play a role in the early development of some sympathetic neurons. ER -