RT Journal Article
SR Electronic
T1 Neuropeptide induction of cyclic GMP increases in the insect CNS: resolution at the level of single identifiable neurons
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 7704
OP 7712
DO 10.1523/JNEUROSCI.14-12-07704.1994
VO 14
IS 12
A1 J Ewer
A1 J De Vente
A1 JW Truman
YR 1994
UL http://www.jneurosci.org/content/14/12/7704.abstract
AB In insects, the neuropeptide eclosion hormone (EH) acts on the CNS to evoke the stereotyped behaviors that cause ecdysis, the shedding of the cuticle at the end of each molt. Concomitantly, EH induces an increase in cyclic GMP (cGMP). Using antibodies against this second messenger, we show that this increase is confined to a network of 50 peptidergic neurons distributed throughout the CNS. Increases appeared 30 min after EH treatment, spread rapidly throughout these neurons, and were extremely long lived. We show that this response is synaptically driven, and does not involve the soluble, nitric oxide (NO)-activated, guanylate cyclase. Stereotyped variations in the duration of the cGMP response among neurons suggest a role in coordinating responses having different latencies and durations.