PT  - JOURNAL ARTICLE
AU  - N Stella
AU  - M Tence
AU  - J Glowinski
AU  - J Premont
TI  - Glutamate-evoked release of arachidonic acid from mouse brain astrocytes
AID  - 10.1523/JNEUROSCI.14-02-00568.1994
DP  - 1994 Feb 01
TA  - The Journal of Neuroscience
PG  - 568--575
VI  - 14
IP  - 2
4099  - http://www.jneurosci.org/content/14/2/568.short
4100  - http://www.jneurosci.org/content/14/2/568.full
SO  - J. Neurosci.1994 Feb 01; 14
AB  - Brain astrocytes in primary culture from the rat or the mouse have been shown to possess ionotropic and metabotropic glutamatergic receptors. The activation of both types of receptors is responsible for a rise in the cytosolic concentration of calcium, while the stimulation of metabotropic receptors induces the accumulation of inositol phosphates. In the present study, it is demonstrated that in striatal astrocytes from mouse embryos, glutamate evokes a release of arachidonic acid. The nonionotropic receptors involved in this effect appeared to be pharmacologically distinct from those coupled to phospholipase C: (1) glutamate displayed different dose-response curves for the production of inositol phosphates (biphasic: EC50 = 25 and 300 microM) and the release of arachidonic acid (monophasic: EC50 = 200 microM); (2) L(+)-2- amino-4-phosphonobutyric acid (AP4) only antagonized the glutamate- evoked release of arachidonic acid without altering the production of inositol phosphates; (3) when used at a concentration of 0.1 mM, quisqualate induced a higher formation of inositol phosphates than glutamate (2 mM) while, in contrast to glutamate, it only weakly stimulated arachidonic acid release when used either at 0.1 mM or 1 mM. L(+)-2-amino-3-phosphonopropionic acid (AP3) suppressed both responses. The glutamate-evoked release of arachidonic acid seems to be oppositely regulated by protein kinases A and C. Indeed, the stimulation of adenylate cyclase by the beta-adrenergic agonist isoproterenol, vasoactive intestinal peptide, or pretreatment of striatal astrocytes with cholera toxin decreased the glutamate-evoked release of arachidonic acid. In contrast, ATP, which markedly stimulated inositol phosphate production, strongly potentiated the glutamate-evoked release of arachidonic acid.(ABSTRACT TRUNCATED AT 250 WORDS)