TY - JOUR T1 - Omega-conotoxin sensitivity and presynaptic inhibition of glutamatergic sensory neurotransmission in vitro JF - The Journal of Neuroscience JO - J. Neurosci. SP - 2800 LP - 2808 DO - 10.1523/JNEUROSCI.14-05-02800.1994 VL - 14 IS - 5 AU - W Gruner AU - LR Silva Y1 - 1994/05/01 UR - http://www.jneurosci.org/content/14/5/2800.abstract N2 - Synaptic transmission between embryonic chick dorsal root ganglion (DRG) neurons and spinal cord neurons was studied in dissociated cell culture. Stimulation of DRG neurons evoked monosynaptic and polysynaptic excitatory responses in the spinal neurons. These responses could be reversibly blocked by application of 6-cyano-7- nitroquinoxaline-2,3-dione (a selective non-NMDA receptor antagonist) and irreversibly eliminated through the presynaptic action of omega- conotoxin GVIA (a selective N-type calcium channel antagonist). As N- type calcium channels in DRG neuron somata are targets for modulation via GABAB receptors, we tested the role of these receptors as regulators of synaptic transmission. Baclofen (a selective GABAB receptor agonist) reversibly inhibited synaptic transmission via a presynaptic, pertussis toxin-sensitive mechanism; CGP 35348 (a selective GABAB receptor antagonist) blocked the actions of baclofen. Taken together, these results demonstrate that N-type calcium channels play a dominant role in glutamatergic sensory neurotransmission. They suggest, in addition, that modulation of N-channel activity may underlie, at least in part, presynaptic inhibition of synaptic transmission between DRG neurons and their targets in the intact spinal cord. ER -