PT  - JOURNAL ARTICLE
AU  - D Weinshenker
AU  - G Garriga
AU  - JH Thomas
TI  - Genetic and pharmacological analysis of neurotransmitters controlling egg laying in C. elegans
AID  - 10.1523/JNEUROSCI.15-10-06975.1995
DP  - 1995 Oct 01
TA  - The Journal of Neuroscience
PG  - 6975--6985
VI  - 15
IP  - 10
4099  - http://www.jneurosci.org/content/15/10/6975.short
4100  - http://www.jneurosci.org/content/15/10/6975.full
SO  - J. Neurosci.1995 Oct 01; 15
AB  - We have investigated the neurotransmitters used to control egg-laying in C. elegans. Previous studies suggested that 5-HT released by the HSN motor neurons stimulates egg laying, and that tricyclic antidepressants potentiate egg laying by blocking reuptake of 5-HT by the HSN neurons. We report studies of the wild type and a mutant that lacks detectable 5- HT that suggest 5-HT is not required for egg-laying. Furthermore, we find that ACh is required for egg laying in response to 5-HT, suggesting that 5-HT is not sufficient to activate egg laying. The dominant egl-2(n693) mutation, which causes animals to lay eggs in response to tricyclics but not 5-HT, also conflicts with the model for egg laying. Experiments in which the HSN neurons or 5-HT are removed from egl-2 animals indicate that the action of tricyclics cannot be explained by a block of 5-HT reuptake. We find that D2 family dopamine antagonists can also induce egg laying in egl-2(n693) mutants, and that dopamine inhibits egg laying in the wild type. These results suggest that dominant egl-2 mutations activate an inhibitory dopaminergic pathway that can be blocked by tricyclics and D2 antagonists. We also find that these drugs stimulate egg laying in mutants lacking 5-HT or the HSN neurons, consistent with a target on the egg-laying muscles. In contrast to tricyclics, fluoxetine and other selective 5-HT reuptake inhibitors appear to be specific for 5-HT reuptake in C. elegans egg laying.