RT Journal Article SR Electronic T1 Fibroblast growth factor 2 increases Otx2 expression in precursor cells from mammalian telencephalon JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 7879 OP 7891 DO 10.1523/JNEUROSCI.15-12-07879.1995 VO 15 IS 12 A1 L Robel A1 M Ding A1 AJ James A1 X Lin A1 A Simeone A1 JF Leckman A1 FM Vaccarino YR 1995 UL http://www.jneurosci.org/content/15/12/7879.abstract AB Dissociated primary cultures from rat telencephalon at different developmental stages were used to study the effect of basic fibroblast growth factor (FGF2) on Otx2, Dlx1, and Emx1, three homeobox genes expressed in different regions of the developing mammalian forebrain. At embryonic day (E)13.5. the regional pattern of expression of Otx1, Otx2, Dlx1, Dlx2, Dlx5, and Emx1 is maintained in primary culture, suggesting that cells are already committed to a regional identity at this stage. In these cultures, Otx2 is expressed by precursor cells, whereas Dlx1 and Emx1 are predominantly expressed by postmitotic cells. We found that FGF2 increased Otx2 expression within precursor cells and the total number of Otx2-expressing cells. This effect was gene- specific, dose-dependent, and temporally regulated, with larger effects at earlier stages of development (E11.5). At E13.5, the effect of FGF2 on Otx2 expression was restricted to the basal telencephalon. Our results suggest that a restricted population of neuroblasts respond to FGF2 in a temporally regulated fashion by proliferating and increasing Otx2 expression. This interaction between FGF2 and Otx2 may be important for the regulation of neurogenesis in the forebrain.