PT  - JOURNAL ARTICLE
AU  - Doris Nonner
AU  - Ellen F. Barrett
AU  - John N. Barrett
TI  - Neurotrophin Effects on Survival and Expression of Cholinergic Properties in Cultured Rat Septal Neurons under Normal and Stress Conditions
AID  - 10.1523/JNEUROSCI.16-21-06665.1996
DP  - 1996 Nov 01
TA  - The Journal of Neuroscience
PG  - 6665--6675
VI  - 16
IP  - 21
4099  - http://www.jneurosci.org/content/16/21/6665.short
4100  - http://www.jneurosci.org/content/16/21/6665.full
SO  - J. Neurosci.1996 Nov 01; 16
AB  - These studies tested the hypothesis that survival-promoting effects of neurotrophins on basal forebrain cholinergic neurons are enhanced under stress. Septal neurons from embryonic day 14–15 rats exposed for 10–14 d to neurotrophin [nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), or neurotrophin-4 (NT-4), each at 100 ng/ml] showed a two- to threefold increase in choline acetyltransferase (ChAT) activity, with little evidence of synergistic interactions. Neurotrophins produced no significant increase in the survival of total or acetylcholinesterase (AChE)-positive neurons at moderate plating density (1200–1600 cells/mm2). However, with very low plating densities (2–28 cells/mm2) BDNF, NT-3, and NT-4 (but not NGF) increased total neuronal survival, and BDNF increased survival of AChE-positive neurons.NGF and BDNF enhanced ChAT activity and survival of cholinergic neurons after a 24 hr hypoglycemic stress, even when added 1 hr after stress onset. All four tested neurotrophins increased total neuronal survival after hypoglycemic stress. These results suggest that neurotrophins are important for preservation of central cholinergic function under stress conditions, with different neurotrophins protecting against different stresses. The stress-associated survival-promoting effects of neurotrophins were not limited to the cholinergic subpopulation.