PT  - JOURNAL ARTICLE
AU  - Guibao Gu
AU  - Anthony A. Rojo
AU  - Michele C. Zee
AU  - Jianhua Yu
AU  - Richard B. Simerly
TI  - Hormonal Regulation of CREB Phosphorylation in the Anteroventral Periventricular Nucleus
AID  - 10.1523/JNEUROSCI.16-09-03035.1996
DP  - 1996 May 01
TA  - The Journal of Neuroscience
PG  - 3035--3044
VI  - 16
IP  - 9
4099  - http://www.jneurosci.org/content/16/9/3035.short
4100  - http://www.jneurosci.org/content/16/9/3035.full
SO  - J. Neurosci.1996 May 01; 16
AB  - The anteroventral periventricular nucleus (AVPV) is a nodal point in neural circuits regulating secretion of gonadotropin and contains sexually dimorphic populations of hormonally regulated dopamine-, dynorphin-, and enkephalin-containing neurons. Because the tyrosine hydroxylase (TH), prodynorphin (PDYN), and proenkephalin (PENK) genes contain cAMP response elements that control their expression in their promoters, we used histochemical methods to determine whether ovarian steroids alter expression of the cAMP response element-binding protein (CREB) in the AVPV. Because the ability of CREB to activate transcription depends on phosphorylation at Ser133, we also evaluated the effects of acute steroid treatment on levels of phosphorylated CREB (pCREB) in AVPV neurons by using an antibody that differentiates between CREB and pCREB. Treatment of ovariectomized rats with estradiol treatments caused a significant induction in the number of pCREB-immunoreactive nuclei within 30 min that was maintained for at least 4 hr, but did not alter CREB immunostaining in the AVPV. Pretreatment with the estrogen antagonist Nafoxidine blocked this induction. In contrast, acute administration of progesterone to estrogen-primed animals suppressed and then increased pCREB staining in the AVPV at 30 and 60 min, respectively; no significant differences between experimental and control animals were apparent by 2 hr after progesterone treatment. Double-labeling experiments showed that pCREB was colocalized with PDYN, PENK, or TH mRNA in the AVPV, suggesting that pCREB may mediate the effect of steroid hormones on gene expression in these neurons.