PT - JOURNAL ARTICLE AU - Yosky Kataoka AU - Hiroshi Morii AU - Yasuyoshi Watanabe AU - Harunori Ohmori TI - A Postsynaptic Excitatory Amino Acid Transporter with Chloride Conductance Functionally Regulated by Neuronal Activity in Cerebellar Purkinje Cells AID - 10.1523/JNEUROSCI.17-18-07017.1997 DP - 1997 Sep 15 TA - The Journal of Neuroscience PG - 7017--7024 VI - 17 IP - 18 4099 - http://www.jneurosci.org/content/17/18/7017.short 4100 - http://www.jneurosci.org/content/17/18/7017.full SO - J. Neurosci.1997 Sep 15; 17 AB - Excitatory amino acid (EAA) neurotransmitters induce postsynaptic depolarization by activating receptor-mediated cation conductances, a process known to underlie changes in synaptic efficacy. Using a patch-clamp method, we demonstrate here an EAA-dependent postsynaptic anion conductance mediated by EAA transporters present on cerebellar Purkinje cell bodies and dendrites in culture. This transporter-mediated current was modulated by neuronal activity: it exhibited facilitation for >20 min after transient depolarization accompanied by Ca2+ influx. Evidence is presented suggesting that the transporter facilitation is mediated by arachidonate release after Ca2+-dependent activation of phospholipase A2, which exists in Purkinje cells. This postsynaptic reuptake system may represent a novel modulatory mechanism of synaptic transmission as well as prevent neuronal excitotoxicity.