RT Journal Article
SR Electronic
T1 Structural Features Determining Differential Receptor Regulation of Neuronal Ca Channels
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 3689
OP 3698
DO 10.1523/JNEUROSCI.18-10-03689.1998
VO 18
IS 10
A1 Arthur A. Simen
A1 Richard J. Miller
YR 1998
UL http://www.jneurosci.org/content/18/10/3689.abstract
AB Dihydropyridine-insensitive Ca channels are subject to direct receptor G-protein-mediated inhibition to differing extents. α1B channels are much more strongly modulated than α1E channels. To understand the structural basis for this difference, we have constructed and expressed various α1Band α1E chimeric Ca channels and examined their regulation by κ-opioid receptors. Replacement of the first membrane-spanning domain of α1E with the corresponding region of α1B resulted in a chimeric Ca channel that was modulated by κ-opioid receptors to a significantly greater extent than α1E. Transfer of the N terminus and I/II loop from α1B in addition to domain I resulted in a chimeric channel that was modulated to the same extent as α1B. Other regions of the molecule do not appear to contribute significantly to the degree of inhibition obtained, although the C terminus may contribute to facilitation.