%0 Journal Article %A Toby N. Behar %A Anne E. Schaffner %A Catherine A. Scott %A Casey O’Connell %A Jeffery L. Barker %T Differential Response of Cortical Plate and Ventricular Zone Cells to GABA as a Migration Stimulus %D 1998 %R 10.1523/JNEUROSCI.18-16-06378.1998 %J The Journal of Neuroscience %P 6378-6387 %V 18 %N 16 %X A microdissection technique was used to separate differentiated cortical plate (cp) cells from immature ventricular zone cells (vz) in the rat embryonic cortex. The cp population contained >85% neurons (TUJ1+), whereas the vz population contained ∼60% precursors (nestin+ only). The chemotropic response of each population was analyzed in vitro, using an established microchemotaxis assay. Micromolar GABA (1–5 μm) stimulated the motility of cp neurons expressing glutamic acid decarboxylase (GAD), the rate-limiting enzyme in GABA synthesis. In contrast, femtomolar GABA (500 fm) directed a subset of GAD− vz neurons to migrate. Thus, the two GABA concentrations evoked the motility of phenotypically distinct populations derived from different anatomical regions. Pertussis toxin (PTX) blocked GABA-induced migration, indicating that chemotropic signals involve G-protein activation. Depolarization by micromolar muscimol, elevated [K+]o, or micromolar glutamate arrested migration to GABA or GABA mimetics, indicating that migration is inhibited in the presence of excitatory stimuli. These results suggest that GABA, a single ligand, can promote motility via G-protein activation and arrest attractant-induced migration via GABAA receptor-mediated depolarization. %U https://www.jneurosci.org/content/jneuro/18/16/6378.full.pdf