TY - JOUR T1 - Evidence for Opening of Hair-Cell Transducer Channels after Tip-Link Loss JF - The Journal of Neuroscience JO - J. Neurosci. SP - 6748 LP - 6756 DO - 10.1523/JNEUROSCI.18-17-06748.1998 VL - 18 IS - 17 AU - Jens Meyer AU - David N. Furness AU - Hans-Peter Zenner AU - Carole M. Hackney AU - Anthony W. Gummer Y1 - 1998/09/01 UR - http://www.jneurosci.org/content/18/17/6748.abstract N2 - The mechanosensitive transducer channels of hair cells have long been proposed to be gated directly by tension in the tip links. These are thin, elastic extracellular elements connecting the tips of adjacent stereocilia located on the apical surface of the cell. If this hypothesis is true, the channels should close after destruction of tip links. The hypothesis was tested pharmacologically using receptor currents obtained in response to mechanical stimulation of the stereociliary bundle of outer hair cells isolated from the adult guinea pig cochlea. Application of elastase (20 U/ml) or 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetra-acetic acid (BAPTA; 5 mm), both of which are known to disrupt tip links in other hair-cell preparations, led to the expected irreversible loss of receptor currents. However, the cells then displayed a maintained inward current, implying that channels were left permanently open. This current was similar in magnitude to the receptor current before treatment and was reduced reversibly by known blockers of mechanosensitive channels, namely, dihydrostreptomycin (100 μm), amiloride (300 μm), and gadolinium ions (1 mm). These observations suggest that the maintained current flows through the mechanosensitive channels. Electron microscopical analysis of isolated hair cells, exposed to the same concentrations of elastase or BAPTA as in the electrophysiological experiments, demonstrated an almost total loss of tip links in hair bundles that showed no evidence of other mechanical damage. It is concluded that although the tip links are required for mechanoelectrical transduction, the channels are not gated directly by the tip links. ER -