PT - JOURNAL ARTICLE AU - Sean G. Carriedo AU - Hong Zhen Yin AU - Stefano L. Sensi AU - John H. Weiss TI - Rapid Ca<sup>2+</sup> Entry through Ca<sup>2+</sup>-Permeable AMPA/Kainate Channels Triggers Marked Intracellular Ca<sup>2+</sup>Rises and Consequent Oxygen Radical Production AID - 10.1523/JNEUROSCI.18-19-07727.1998 DP - 1998 Oct 01 TA - The Journal of Neuroscience PG - 7727--7738 VI - 18 IP - 19 4099 - http://www.jneurosci.org/content/18/19/7727.short 4100 - http://www.jneurosci.org/content/18/19/7727.full SO - J. Neurosci.1998 Oct 01; 18 AB - The widespread neuronal injury that results after brief activation of highly Ca2+-permeable NMDA channels may, in large part, reflect mitochondrial Ca2+ overload and the consequent production of injurious oxygen radicals. In contrast, AMPA/kainate receptor activation generally causes slower toxicity, and most studies have not found evidence of comparable oxygen radical production. Subsets of central neurons, composed mainly of GABAergic inhibitory interneurons, express AMPA/kainate channels that are directly permeable to Ca2+ ions. Microfluorometric techniques were performed by using the oxidation-sensitive dye hydroethidine (HEt) to determine whether the relatively rapid Ca2+ flux through AMPA/kainate channels expressed on GABAergic neurons results in oxygen radical production comparable to that triggered by NMDA. Consistent with previous studies, NMDA exposures triggered increases in fluorescence in most cultured cortical neurons, whereas high K+ (50 mm) exposures (causing depolarization-induced Ca2+influx through voltage-sensitive Ca2+ channels) caused little fluorescence change. In contrast, kainate exposure caused fluorescence increases in a distinct subpopulation of neurons; immunostaining for glutamate decarboxylase revealed the responding neurons to constitute mainly the GABAergic population. The effect of NMDA, kainate, and high K+ exposures on oxygen radical production paralleled the effect of these exposures on intracellular Ca2+ levels when they were monitored with the low-affinity Ca2+-sensitive dye fura-2FF, but not with the high-affinity dye fura-2. Inhibition of mitochondrial electron transport with CN− or rotenone almost completely blocked kainate-triggered oxygen radical production. Furthermore, antioxidants attenuated neuronal injury resulting from brief exposures of NMDA or kainate. Thus, as with NMDA receptor activation, rapid Ca2+ influx through Ca2+-permeable AMPA/kainate channels also may result in mitochondrial Ca2+ overload and consequent injurious oxygen radical production.