RT Journal Article SR Electronic T1 Spinal Cord Neuronal Precursors Generate Multiple Neuronal Phenotypes in Culture JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 7856 OP 7868 DO 10.1523/JNEUROSCI.18-19-07856.1998 VO 18 IS 19 A1 Anjali J. Kalyani A1 David Piper A1 Tahmina Mujtaba A1 Mary T. Lucero A1 Mahendra S. Rao YR 1998 UL http://www.jneurosci.org/content/18/19/7856.abstract AB Neuronal restricted precursors (NRPs) (Mayer-Proschel et al., 1997) can generate multiple neurotransmitter phenotypes during maturation in culture. Undifferentiated E-NCAM+ (embryonic neural cell adhesion molecule) immunoreactive NRPs are mitotically active and electrically immature, and they express only a subset of neuronal markers. Fully mature cells are postmitotic, process-bearing cells that are neurofilament-M and synaptophysin immunoreactive, and they synthesize and respond to different subsets of neurotransmitter molecules. Mature neurons that synthesize and respond to glycine, glutamate, GABA, dopamine, and acetylcholine can be identified by immunocytochemistry, RT-PCR, and calcium imaging in mass cultures. Individual NRPs also generate heterogeneous progeny as assessed by neurotransmitter response and synthesis, demonstrating the multipotent nature of the precursor cells.Differentiation can be modulated by sonic hedgehog (Shh) and bone morphogenetic protein (BMP)-2/4 molecules. Shh acts as a mitogen and inhibits differentiation (including cholinergic differentiation). BMP-2 and BMP-4, in contrast, inhibit cell division and promote differentiation (including cholinergic differentiation). Thus, a single neuronal precursor cell can differentiate into multiple classes of neurons, and this differentiation can be modulated by environmental signals.