%0 Journal Article %A Cornelis P. Tensen %A Kingsley J. A. Cox %A August B. Smit %A Roel C. van der Schors %A Wolfgang Meyerhof %A Dietmar Richter %A Rudi J. Planta %A Petra M. Hermann %A Jan van Minnen %A Wijnand P. M. Geraerts %A Jaco C. Knol %A Julian F. Burke %A Erno Vreugdenhil %A Harm van Heerikhuizen %T The Lymnaea Cardioexcitatory Peptide (LyCEP) Receptor: A G-Protein–Coupled Receptor for a Novel Member of the RFamide Neuropeptide Family %D 1998 %R 10.1523/JNEUROSCI.18-23-09812.1998 %J The Journal of Neuroscience %P 9812-9821 %V 18 %N 23 %X A novel G-protein–coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the molluscLymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106,Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaeabrain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In theLymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEPin vitro. These data confirm that LyCEP is an RFamide ligand for GRL106. %U https://www.jneurosci.org/content/jneuro/18/23/9812.full.pdf