TY - JOUR T1 - Mitochondrial Control of Acute Glutamate Excitotoxicity in Cultured Cerebellar Granule Cells JF - The Journal of Neuroscience JO - J. Neurosci. SP - 10277 LP - 10286 DO - 10.1523/JNEUROSCI.18-24-10277.1998 VL - 18 IS - 24 AU - Roger F. Castilho AU - Oskar Hansson AU - Manus W. Ward AU - Samantha L. Budd AU - David G. Nicholls Y1 - 1998/12/15 UR - http://www.jneurosci.org/content/18/24/10277.abstract N2 - Mitochondria within cultured rat cerebellar granule cells have a complex influence on cytoplasmic free Ca2+([Ca2+]c) responses to glutamate. A decreased initial [Ca2+]celevation in cells whose mitochondria are depolarized by inhibition of the ATP synthase and respiratory chain (conditions which avoid ATP depletion) was attributed to enhanced Ca2+ extrusion from the cell rather than inhibited Ca2+ entry via the NMDA receptor. Even in the presence of elevated extracellular Ca2+, when [Ca2+]cresponses were restored to control values, such cells showed resistance to acute excitotoxicity, defined as a delayed cytoplasmic Ca2+ deregulation (DCD) during glutamate exposure. DCD was a function of the duration of mitochondrial polarization in the presence of glutamate rather than the total period of glutamate exposure. Once initiated, DCD could not be reversed by NMDA receptor inhibition. In the absence of ATP synthase inhibition, respiratory chain inhibitors produced an immediate Ca2+deregulation (ICD), ascribed to an ATP deficit. In contrast to DCD, ICD could be reversed by subsequent ATP synthase inhibition with or without additional NMDA receptor blockade. DCD could not be ascribed to the failure of an ATP yielding metabolic pathway. It is concluded that mitochondria can control Ca2+ extrusion from glutamate-exposed granule cells by the plasma membrane in three ways: by competing with efflux pathways for Ca2+, by restricting ATP supply, and by inducing a delayed failure of Ca2+ extrusion. Inhibitors of the mitochondrial permeability transition only marginally delayed the onset of DCD. ER -