PT  - JOURNAL ARTICLE
AU  - Gwendolyn T. Wong
AU  - Luis Ruiz-Avila
AU  - Robert F. Margolskee
TI  - Directing Gene Expression to Gustducin-Positive Taste Receptor Cells
AID  - 10.1523/JNEUROSCI.19-14-05802.1999
DP  - 1999 Jul 15
TA  - The Journal of Neuroscience
PG  - 5802--5809
VI  - 19
IP  - 14
4099  - http://www.jneurosci.org/content/19/14/5802.short
4100  - http://www.jneurosci.org/content/19/14/5802.full
SO  - J. Neurosci.1999 Jul 15; 19
AB  - We have demonstrated that an 8.4 kb segment (GUS8.4) from the upstream region of the mouse α-gustducin gene acts as a fully functional promoter to targetlacZ transgene expression to the gustducin-positive subset of taste receptor cells (TRCs). The GUS8.4 promoter drove TRC expression of the β-galactosidase marker at high levels and in a developmentally appropriate pattern. The gustducin minimal 1.4 kb promoter (GUS1.4) by itself was insufficient to specify TRC expression. We also identified an upstream enhancer from the distal portion of the murine gustducin gene that, in combination with the minimal promoter, specified TRC expression of transgenes. Expression of the lacZ transgene from the GUS8.4 promoter and of endogenous gustducin was coordinately lost after nerve section and simultaneously recovered after reinnervation, confirming the functionality of this promoter. Transgenic expression of rat α-gustducin restored responsiveness of gustducin null mice to both bitter and sweet compounds, demonstrating the utility of the gustducin promoter.