@article {Ruchhoeft8454, author = {Maureen L. Ruchhoeft and Shin-ichi Ohnuma and Lisa McNeill and Christine E. Holt and William A. Harris}, title = {The Neuronal Architecture of Xenopus Retinal Ganglion Cells Is Sculpted by Rho-Family GTPases In Vivo}, volume = {19}, number = {19}, pages = {8454--8463}, year = {1999}, doi = {10.1523/JNEUROSCI.19-19-08454.1999}, publisher = {Society for Neuroscience}, abstract = {Dendritogenesis, axonogenesis, pathfinding, and target recognition are all affected in distinct ways when Xenopus retinal ganglion cells (RGCs) are transfected with constitutively active (ca), wild-type (wt), and dominant negative (dn) Rho-family GTPases in vivo. Dendritogenesis required Rac1 and Cdc42 activity. Moreover, ca-Rac1 caused dendrite hyperproliferation. Axonogenesis, in contrast, was inhibited by ca-Rac1. This phenotype was partially rescued by the coexpression of dn cyclin-dependent kinase (Cdk5), a proposed effector of Rac1, suggesting that Rac1 activity must be regulated tightly for normal axonogenesis. Growth cone morphology was particularly sensitive to dn-RhoA and wt-Cdc42 constructs. These also caused targeting errors, such as tectal bypass, suggesting that cytoskeletal rearrangements are involved in target recognition and are transduced by these pathways.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/19/19/8454}, eprint = {https://www.jneurosci.org/content/19/19/8454.full.pdf}, journal = {Journal of Neuroscience} }