PT  - JOURNAL ARTICLE
AU  - Sean P. Cregan
AU  - Jason G. MacLaurin
AU  - Constance G. Craig
AU  - George S. Robertson
AU  - Donald W. Nicholson
AU  - David S. Park
AU  - Ruth S. Slack
TI  - Bax-Dependent Caspase-3 Activation Is a Key Determinant in p53-Induced Apoptosis in Neurons
AID  - 10.1523/JNEUROSCI.19-18-07860.1999
DP  - 1999 Sep 15
TA  - The Journal of Neuroscience
PG  - 7860--7869
VI  - 19
IP  - 18
4099  - http://www.jneurosci.org/content/19/18/7860.short
4100  - http://www.jneurosci.org/content/19/18/7860.full
SO  - J. Neurosci.1999 Sep 15; 19
AB  - p53 is a pivotal molecule regulating the death of neurons both after acute injury and during development. The molecular mechanisms by which p53 induces apoptosis in neuronal cells, however, are not well understood. We have shown previously that adenovirus-mediated p53 gene delivery to neurons was sufficient to induce apoptosis. In the present study we have examined the molecular mechanism by which p53 evokes neuronal cell death. Adenovirus-mediated delivery of p53 to cerebellar granule neurons resulted in caspase-3 (CPP32) activation followed by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) staining and loss of viability as determined by an MTT survival assay. To determine whether Bax is essential for caspase-3 activation, p53 was expressed in Bax-deficient cells. Bax null neurons did not exhibit caspase-3 activation in response to p53 and were protected from apoptosis. To determine whether Bax-dependent caspase-3 activation was required in p53-mediated neuronal cell death, caspase-3-deficient neurons were examined. Our results indicate that caspase-3-deficient neurons exhibit a remarkable delay in apoptosis and a dramatic decrease in TUNEL-positive cells. These studies demonstrate that p53-induced cell death in postmitotic neurons involves a Bax-dependent caspase-3 activation, suggesting that these molecules are important determinants in neuronal cell death after injury.